What is the major issue of this paper?

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What is the major issue of this paper? How C. elegans embryos divide? How germline specification is achieved? What is PIE-1 protein? Where is pie-1 mRNA localized? How germline blastomeres divide?

How did the authors clone the pie-1 gene? Through Antibody staining Tc1 transposon insertion or tagging combined with genetic mapping C. elegans lineage analysis They cloned the gene by pure luck They cloned the gene by a candidate approach

What is the most important concept that you learn from reading this paper? The localization patterns of PIE-1 How pluripotency of a stem cell is maintained by a general repressor What kind of protein PIE-1 is C. elegans is a good animal model for studying germ cell specification PIE-1 associates with centrosomes during cell division

How did the authors reach the conclusion that PIE-1 might be a localized general repressor? A. from antibody staining B. from cell lineage analysis C. from protein sequence D. it is just a wild guess E. from its interactions with other somatic-cell determining genes

What are the important questions that remain to be addressed and need to be analyzed in subsequent study? A. How does PIE-1 function? How PIE-1 is selectively degraded in somatic daughters? How does PIE-1 associate with centrosomes? What are the repression targets of PIE-1? All of the above