S. P Grogan, Ph. D. , F Rieser, B. Sc

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A static, closed and scaffold-free bioreactor system that permits chondrogenesis in vitro S.P Grogan, Ph.D., F Rieser, B.Sc., V Winkelmann, HFP, S Berardi, Ph.D., P Mainil-Varlet, M.D., Ph.D. Osteoarthritis and Cartilage Volume 11, Issue 6, Pages 403-411 (June 2003) DOI: 10.1016/S1063-4584(03)00053-0

Fig. 1 (A) The DeNovo™ closed chamber system. (I) The top and base that supports the (II) inner chambers, which come in two inner diameter sizes. (III) Base support with membrane and inner chamber. The membrane is held in place between the lower base and the inner chamber. Cells are then seeded into this space, followed by an upper membrane that is then held in place by adding the top support. (IV) A completely constructed closed chamber system with the upper and lower membranes in place. (B) Mechanism of neo-cartilage formation. The cells are seeded at high density between semi-permeable membranes that protect the developing construct from turbulence and the loss of new matrix-associated molecules during media changes. Osteoarthritis and Cartilage 2003 11, 403-411DOI: (10.1016/S1063-4584(03)00053-0)

Fig. 2 (A) Macrophotograph of neo-cartilage formed after 28 days of culture; (B) confocal live/dead image; (C) alcian blue histology stain; (D) safranin-O histology stain; (E) electron micrograph showing chondrocytes, interterritorial zone formation and matrix deposition; (F) electron micrograph of collagen fibrils and aggregates of proteoglycan. Osteoarthritis and Cartilage 2003 11, 403-411DOI: (10.1016/S1063-4584(03)00053-0)

Fig. 3 SDS-PAGE gel analysis showing collagen subtypes found in engineered neo-cartilage. Osteoarthritis and Cartilage 2003 11, 403-411DOI: (10.1016/S1063-4584(03)00053-0)

Fig. 4 Semi-quantitative RT-PCR showing the expression level (mean±standard error) of selected cartilage specific genes of interest relative to GAPDH expression. Osteoarthritis and Cartilage 2003 11, 403-411DOI: (10.1016/S1063-4584(03)00053-0)