Hemodialysis Modulates Gene Expression Profile in Skeletal Muscle Vallabh O. Shah, PhD, Elizabeth A. Dominic, Pope Moseley, MD, Gavin Pickett, Margret Fleet, MD, Scott Ness, PhD, Dominic S.C. Raj, MD, DM  American Journal of Kidney Diseases  Volume 48, Issue 4, Pages 616-628 (October 2006) DOI: 10.1053/j.ajkd.2006.05.032 Copyright © 2006 National Kidney Foundation, Inc. Terms and Conditions

Fig 1 Cluster of genes regulated by HD in skeletal muscle. Columns indicate subjects and condition (pre-HD or post-HD) in which the sample was obtained. For each probe set, the normalized expression of each subject represents the number of SDs from the mean. The intensity of each block represents the magnitude of difference from the mean. Red indicates upregulated genes, and blue, downregulated gene. Results show that 22 genes were upregulated in muscle and 1gene (TOB1) was downregulated during HD. Data were analyzed through the use of Ingenuity Pathways Analysis (Ingenuity(r) Systems, www.ingenuity.com). American Journal of Kidney Diseases 2006 48, 616-628DOI: (10.1053/j.ajkd.2006.05.032) Copyright © 2006 National Kidney Foundation, Inc. Terms and Conditions

Fig 2 Network of genes modulated by HD. Shaded genes were identified by means of microarray analysis, and others were associated with regulated genes based on pathway analysis. Meanings of node shapes are indicated. (A) Eleven genes associated with the inflammation network were upregulated, and 1 gene (TOB1) was downregulated. (B) Nine genes related to the apoptosis network showed increased expression during HD. American Journal of Kidney Diseases 2006 48, 616-628DOI: (10.1053/j.ajkd.2006.05.032) Copyright © 2006 National Kidney Foundation, Inc. Terms and Conditions