Supplementation with estradiol-17β improves porcine oocyte maturation and subsequent embryo development Ji-Su Kim, Ph.D., Bong-Seok Song, Ph.D., Sang-Rae.

Slides:

Advertisements

Similar presentations

Relationships Between Capsular Stiffness and Clinical Features in Adhesive Capsulitis of the Shoulder Sang Yoon Lee, MD, Kyu Jin Lee, PhD, Won Kim, MD,

Advertisements

Susannah Baruch, J.D., David Kaufman, Ph.D., Kathy L. Hudson, Ph.D.

Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (≥10 cells), bringing into question current embryological.

Multiple gestation associated with infertility therapy: an American Society for Reproductive Medicine Practice Committee opinion Fertility and Sterility

Wendy S. Vitek, M. D. , Jeannine Witmyer, Ph. D. , Sandra A. Carson, M

Impact of blastocyst transfer on offspring sex ratio and the monozygotic twinning rate: a systematic review and meta-analysis Hye Jin Chang, M.D., Ph.D.,

Benefit of uterine artery ligation in laparoscopic myomectomy

Gene expression analysis of a new source of human oocytes and embryos for research and human embryonic stem cell derivation Sharon F. Sneddon, Ph.D.,

Susannah Baruch, J.D., David Kaufman, Ph.D., Kathy L. Hudson, Ph.D.

Leukemia inhibitory factor induces cumulus expansion in immature human and mouse oocytes and improves mouse two-cell rate and delivery rates when it is.

Successful use of magnetic resonance–guided focused ultrasound surgery to relieve symptoms in a patient with symptomatic focal adenomyosis Sang-Wook.

Fertility and Sterility Volume 97, Issue 3, Pages (March 2012)

MRNA expression pattern of insulin-like growth factor components of granulosa cells and cumulus cells in women with and without polycystic ovary syndrome.

Sang-Hee Yoon, M. D. , Ji Young Lee, M. D. , Soo-Nyung Kim, M. D

Michael M. Alper, M.D. Fertility and Sterility

Composition of single-step media used for human embryo culture

Effect of a very-low-calorie diet on in vitro fertilization outcomes

Adherence compounds in embryo transfer media for assisted reproductive technologies: summary of a Cochrane review Stephan Bontekoe, M.D., Neil Johnson,

Zhi-Yong Yang, M.D., Ri-Cheng Chian, Ph.D. Fertility and Sterility

Follicular fluid anti-Müllerian hormone and inhibin B concentrations: comparison between gonadotropin-releasing hormone (GnRH) agonist and GnRH antagonist.

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Walid E. Maalouf, Ph. D. , Mina N. Mincheva, M. Med. Sc. , Bruce K

Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc.,

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Live birth of twins derived from zona-free oocytes

Complex chromosomal rearrangements in infertile males: complexity of rearrangement affects spermatogenesis Ji Won Kim, M.D., Eun Mi Chang, M.D., Seung-Hun.

Iris G. Insogna, M. D. , Malinda S. Lee, M. D. , M. B. A. , Rebecca M

Shi Ying Jin, Ph. D. , Lei Lei, Ph. D. , Ariella Shikanov, Ph. D

Selection of patients for natural cycle in vitro fertilization combined with in vitro maturation of immature oocytes Jin-Ho Lim, M.D., Seong-Ho Yang,

Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence Ze-Xu Jiao, M.D., Ph.D., Min Xu,

Martin D. Keltz, M. D. , Josh C. Skorupski, M. D. , Katrina Bradley, M

Oocyte cryopreservation

Complex chromosomal rearrangements in infertile males: complexity of rearrangement affects spermatogenesis Ji Won Kim, M.D., Eun Mi Chang, M.D., Seung-Hun.

Mieke Carine Wim Eeckhaut, Ph.D. Fertility and Sterility

A healthy live birth after successful preimplantation genetic diagnosis for carriers of complex chromosome rearrangements Chun Kyu Lim, M.S., Jae Won.

Clomiphene citrate at 50: the dawning of assisted reproduction

Introduction Fertility and Sterility

Immature oocyte retrieval and in vitro oocyte maturation at different phases of the menstrual cycle in women with cancer who require urgent gonadotoxic.

Ongoing twin pregnancy after rescue intracytoplasmic sperm injection of unfertilized abnormal oocytes Navid Esfandiari, D.V.M., Ph.D., H.C.L.D., E. Anne.

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Nitric oxide metabolite production in the human preimplantation embryo and successful blastocyst formation Christopher W. Lipari, M.D., Jairo E. Garcia,

Poor sperm quality affects clinical outcomes of intracytoplasmic sperm injection in fresh and subsequent frozen–thawed cycles: potential paternal effects.

Prospective cohort study of three- versus two-dimensional ultrasound for prediction of oocyte maturity Doron Shmorgun, M.D., Edward Hughes, M.D., Patrick.

Live babies born per oocyte retrieved in a subpopulation of oocyte donors with repetitive reproductive success J. Ryan Martin, M.D., Jason G. Bromer,

Ri-Cheng Chian, Ph.D., Peter S. Uzelac, M.D., Geeta Nargund, M.D.

Derivation of developmentally competent oocytes by in vitro culture of preantral follicles retrieved from aged mice Jung Kyu Choi, B.Sc., Jong Il Ahn,

Single-blastomere whole-genome DNA fingerprinting results in unequivocal embryo identification – a powerful new clinical and diagnostic tool N.R. Treff,

Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles Giannina.

L. Scott, J. Bernsten, K. DeLegge, J. Hill, N. Ramsing

Mi-Kyung Kim, M. D. , Jung Ryeol Lee, M. D. , Byung Chul Jee, M. D

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in.

Failed IVF cycles and the risk of subsequent preeclampsia or fetal growth restriction: a case-control exploratory study Ioanna Tsoumpou, M.D., Ahmed.

Yong Jin Kim, M. D. , Seung-Yup Ku, M. D. , Ph. D. , Byung Chul Jee, M

Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development Byung Chul Jee, M.D., Hai-Ying Chen,

MicroRNA: key gene expression regulators

Paracrine factors from cumulus-enclosed oocytes ensure the successful maturation and fertilization in vitro of denuded oocytes in the cat model Natasha.

Current evaluation of amenorrhea

Genetic evaluation procedures at sperm banks in the United States

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

An in vivo murine model of rosiglitazone use in pregnancy

Late Stages of Embryo Progression are a Much Better Predictor of Clinical Pregnancy Than Early Cleavage in ICSI and IVF Cycles With Blastocyst-Stage Transfer

Occurrence and developmental consequences of vacuoles throughout preimplantation development Thomas Ebner, Ph.D., Marianne Moser, Ph.D., Michael Sommergruber,

Effectiveness of different routes of misoprostol administration before operative hysteroscopy: a randomized, controlled trial Taejong Song, M.D., Mi.

Se-Jin Yoon, Ph. D. , Deog-Bon Koo, Ph. D. , Jung Sun Park, M. Sc

Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization.

Influence of different oocyte insemination techniques on early and late morphokinetic parameters: retrospective analysis of 500 time-lapse monitored blastocysts

Endometrial polyps affect uterine receptivity

Effect of oocyte quality on blastocyst development after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a sheep model Maria.

Haplotype analysis of the matrix metalloproteinase-9 gene associated with advanced- stage endometriosis You Jung Han, M.D., M.S., Han-Na Kim, M.S., Jin-Kook.

Not all twins are monozygotic after elective single embryo transfer: analysis of 32,600 elective single embryo transfer cycles as reported to the Society.

Presentation transcript:

Supplementation with estradiol-17β improves porcine oocyte maturation and subsequent embryo development Ji-Su Kim, Ph.D., Bong-Seok Song, Ph.D., Sang-Rae Lee, D.V.M., Ph.D., Seung-Bin Yoon, B.Sc., Jae-Won Huh, Ph.D., Sun-Uk Kim, Ph.D., Ekyun Kim, Ph.D., Sang-Hyun Kim, D.V.M., Ph.D., Young-Kug Choo, Ph.D., Deog-Bon Koo, Ph.D., Kyu-Tae Chang, Ph.D. Fertility and Sterility Volume 95, Issue 8, Pages 2582-2584 (June 2011) DOI: 10.1016/j.fertnstert.2011.03.013 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Effects of E2 on oocyte maturation and subsequent early embryogenesis in pigs. (A) Effects of E2 supplementation on meiotic progression of porcine oocytes at 22 and 44 hours of IVM (n = 3). (B, C) Expression level of p34cdc2 and phosphorylation of ERK1/2 in MII oocytes matured in the presence or absence of E2 (n = 3). (D, E) Effects of E2-assisted IVM on pronucleus formation and rates of fertilization and polyspermy after IVF (n = 3). (F, G) Effect of E2-assisted IVM on blastocyst development from IVF- or SCNT-derived porcine embryos (n = 4). (H, I) Effects of E2-assisted IVM on blastomere apoptosis (TUNEL) and the expression of apoptosis-associated genes in IVF- or SCNT-derived blastocysts (n ≥ 80). Data are shown as the mean ± SD. ∗Values differ significantly (P<.05). Fertility and Sterility 2011 95, 2582-2584DOI: (10.1016/j.fertnstert.2011.03.013) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions