The leukocyte activation antigen CD69 limits allergic asthma and skin contact hypersensitivity Pilar Martín, PhD, Manuel Gómez, PhD, Amalia Lamana, PhD,

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Presentation transcript:

The leukocyte activation antigen CD69 limits allergic asthma and skin contact hypersensitivity Pilar Martín, PhD, Manuel Gómez, PhD, Amalia Lamana, PhD, Adela Matesanz Marín, José R. Cortés, PhD, Marta Ramírez-Huesca, Olga Barreiro, PhD, Pedro López-Romero, PhD, Cristina Gutiérrez-Vázquez, Hortensia de la Fuente, MD, PhD, Aránzazu Cruz-Adalia, Francisco Sánchez-Madrid, PhD Journal of Allergy and Clinical Immunology Volume 126, Issue 2, Pages 355-365.e3 (August 2010) DOI: 10.1016/j.jaci.2010.05.010 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 OVA-induced allergic airway inflammation is enhanced in CD69 KO mice. WT and CD69 KO mice were sensitized to OVA, and airway inflammation was induced by aerosol OVA challenge; control animals were challenged with PBS (see Methods). Lung sections were stained with H&E (A) or anti–VCAM-1 antibody revealed with a peroxidase-conjugated secondary (B). C, Percentages of bronchial epithelium staining for VCAM-1. Horizontal lines denote median values (∗P < .01). Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Exacerbated eosinophil recruitment in CD69-deficient mice. A, Absolute numbers of total cells, eosinophils and macrophages in BAL. Results are means (SDs) from 10 mice per genotype. Bonferroni P values from 1-way ANOVA (∗P < .01; ∗∗P < .001). A representative experiment of a total of 4 is shown. B, BAL cell counts of OVA-WT or mice treated with anti-CD69 mAbs (2.2, black bars) or with an isotype control IgG (gray bars). Results are means (SDs) from 6 mice per genotype. C, BAL cells were recovered, stained for CD11c and CD11b and analyzed by flow cytometry. D, Cytospin BAL samples from WT and CD69 KO mice stained with Wright-Giemsa. E, OTII or OTKO mice were adoptively transferred with TH2 plus TH17 cells obtained ex vivo from OVAp-restimulated lymph nodes of OTII-immunized or OTKO-immunized mice. Absolute numbers of the indicated leukocyte population were analyzed in BALs. Results are means (SDs) of 10 mice per genotype. Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 CD69 deficiency increases the levels of TH2 and TH17 cytokines in the lungs of OVA-immunized mice. BAL levels of the cytokines IL-4, IL-5, and IL-13 (A) and IL-17 (B) and of eotaxin and OVA-specific IgE (C) measured by ELISA. Bars represent means (SDs) of 4 (B) or 6 (A and C) mice per genotype. Bonferroni P values from 1-way ANOVA (∗P < .05; ∗∗P < .01). Data are representative of 2 (B) or 4 (A and C) independent experiments. Lungs of WT or CD69 KO mice were collected 48 hours after OVA inhalation. Cells were recovered after lung digestion and stained for CD11c and Gr-1. The percentages of lymphocytes, neutrophils, macrophages, eosinophils, plasmacytoids DCs (pDCs) and conventional DCs (cDCs) were analyzed by fluorescence-activated cell sorting (D). Bars represent means (SDs) of 6 mice per genotype. E, Protein expression of IL-17, IL-4, and GM-CSF, measured by fluorescence-activated cell sorting after suspension bead array, in lung homogenates of WT and CD69 KO mice sensitized and challenged (aerosol) with OVA to induce airway inflammation; control animals were sensitized with OVA and challenged with PBS. Bars represent means (SDs) of 10 mice per genotype. Bonferroni P values from 1-way ANOVA (∗P < .003; ∗∗P < .0001). Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 CD69 deficiency upregulates S1P1 expression on TH17 cells. A, Fluorescence-activated cell sorting analysis of membrane levels of S1P1 and S1P3 in OTII and OTKO TH2 and TH17 cells. An irrelevant polyclonal antibody (black line) was used as a control. The bar chart shows the percentages of S1P1 and S1P3–positive cells; data are means ± SDs from 2 independent experiments. B, Western analysis of S1P1 and S1P3 in membrane and cytosol extracts of naive CD4+ and TH17 T cells from OTII and OTKO mice. The bar charts show densitometry analysis of the ratios of membrane versus cytosolic content of S1P1 in naive and TH17 cells. AU, Arbitrary unit. C, Quantitative RT-PCR analysis of S1P receptor mRNA expression. Results were normalized to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression. Data are representative of 3 experiments. D, OTII and OTKO mice were adoptively transferred with TH2 plus TH17 cells obtained from OVA-immunized OTII or OTKO mice and were exposed to inhaled OVA. The levels of S1P1 and S1P3 from OVAp-restimulated mediastinal lymph node CD4+ T cells were analyzed by flow cytometry. Bars represent means (SDs) of 5 mice per group. Tukey HSD (Honestly Significant Difference) P values from 1-way ANOVA (∗P < .05; ∗∗P < .01). Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 CD69 deficiency enhances the contact hypersensitivity response to oxazolone in mice. A,Left panel, Time course of ear swelling of treated WT and CD69 KO mice (means ± SDs; n = 6). Right panel, Ear swelling at the peak of the CHS response (means ± SDs; n = 36). B, H&E stained 5 μm sections of ears collected 48 hours after OXZ challenge. Bar = 100 μm. C, RT-PCR analysis of cytokine expression in inflamed ear tissue at the peak of inflammation. Results were normalized to GAPDH expression. Bars represent means ± SEMs; n = 4 to 12 mice. D, CHS response in WT mice treated with either anti-CD69 mAb 2.2 or isotype-matched control mAb 2.8. Left panel, Time course of ear swelling (means ± SDs; n = 5). Right panel, Net ear swelling at the peak of the CHS response (means ± SDs; n = 24). ∗P < .01; ∗∗P < .001 (Student t test). Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 IFN-γ T-cell response induced after oxazolone sensitization is enhanced in CD69 KO mice. A, ELISA analysis of IFN-γ secreted by DLN cells from OXZ-sensitized mice activated by plate-bound anti CD3 mAb. B, ELISA analysis of IL-4 (left panel) or IFN-γ (right panel) secreted by CD4+ and CD8+ T cells purified from cell suspensions of DLNs from OXZ sensitized mice and activated by phorbol 12-myristate 13-acetate plus ionomycin. Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 7 Enhanced effector T-cell response induced after oxazolone sensitization increase CHS in CD69 KO mice. A, Flow cytometry analysis of intracellular IL-17 in activated CD4+ and CD8+ T cells from sensitized mice. Representative dot plots (right panel). Percentages of IL17+ cells (left panel). Means ± SDs; n = 18. B, RT-PCR analysis of cytokine expression in DLNs from sensitized mice. Results were normalized to GAPDH expression (means ± SEMs; n = 8 to 17 mice). C, Percentage ear swelling reduction 48 hours after challenge in mice treated with anti–IL-17 neutralizing mAb (IL-17) or isotype matched control mAb. Means ± SDs; n = 5. Adoptive transfer: DLN cells from sensitized WT or CD69 KO mice were injected intravenously into either WT or CD69 KO naive mice that were later challenged with OXZ. See additional information on Methods in the Online Repository at www.jacionline.org. D, Net ear swelling at the indicated times (means ± SDs; n = 5). E, H&E-stained 5-μm sections of ears collected 48 hours after OXZ challenge. Bar = 100 μm. ∗P < .01; ∗∗P < .005 (Student t test). Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

S1P1 expression in OTII Th17 differentiation cultures in the presence of anti-CD69 mAb. A, FACS analysis of membrane levels of S1P1 (blue line) and S1P3 (red line) in OTII Th17 cells cultured in the presence of anti-CD69 mAb 2.2 at the indicated concentrations or with an isotype control Ab (Unspecific Ab). B, FACS analysis of CD69 expression levels in the membrane of OTII Th17 cells cultured as in A or without Abs (Control, grey line). C, FACS analysis of the percentage of positive cells for S1P1, S1P3, CD69 or an isotype control (Unspecific Ab) in OTII Th17 cells cultured as in B. Journal of Allergy and Clinical Immunology 2010 126, 355-365.e3DOI: (10.1016/j.jaci.2010.05.010) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions