Volume 54, Issue 2, Pages (August 1998)

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Volume 54, Issue 2, Pages 530-535 (August 1998) Plasma α2 macroglobulin is increased in nephrotic patients as a result of increased synthesis alone Monique G. M. de Sain-van der Velden, Ton J. Rabelink, Dirk-Jan Reijngoud, Mireille M. Gadellaa, Hieronymus A.M. Voorbij, Frans Stellaard, George A. Kaysen Kidney International Volume 54, Issue 2, Pages 530-535 (August 1998) DOI: 10.1046/j.1523-1755.1998.00018.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 SDS-PAGE (4.5%) pattern of the α2 macroglobulin (α2M) immunoprecipitate from plasma after direct treatment with anti sera. Lane 1: α2M standard, Lane 2: antibody, Lane 3: wide range protein standard (200 kD, 116 kD, 97 kD), Lane 4: α2M precipitate. Kidney International 1998 54, 530-535DOI: (10.1046/j.1523-1755.1998.00018.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Percentage enrichment of valine in the 180 kD subunit in relation to the enrichment in plasma valine between four and six hours of 13C valine infusion. Shown is the mean ± sem curve for both nephrotic patients (○; N = 7) and controls (•; N = 4). Kidney International 1998 54, 530-535DOI: (10.1046/j.1523-1755.1998.00018.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 3 Relationship between the ASR of α 2M (mg/kg/day) and plasma α2M concentration (g/liter) for nephrotic patients (○; N = 7) and controls (•; N = 4). The 95% confidence limits of the whole group are shown on either side of the regression line (r2 =0.821; P = 0.0001; N = 11). Kidney International 1998 54, 530-535DOI: (10.1046/j.1523-1755.1998.00018.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 530-535DOI: (10. 1046/j. 1523-1755. 1998 Kidney International 1998 54, 530-535DOI: (10.1046/j.1523-1755.1998.00018.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 530-535DOI: (10. 1046/j. 1523-1755. 1998 Kidney International 1998 54, 530-535DOI: (10.1046/j.1523-1755.1998.00018.x) Copyright © 1998 International Society of Nephrology Terms and Conditions