Transferable plasmid mediating resistance to multiple antimicrobial agents in Klebsiella pneumoniae isolates in Greece I. Galani, E. Xirouchaki, K. Kanellakopoulou,

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Presentation transcript:

Transferable plasmid mediating resistance to multiple antimicrobial agents in Klebsiella pneumoniae isolates in Greece I. Galani, E. Xirouchaki, K. Kanellakopoulou, G. Petrikkos, H. Giamarellou Clinical Microbiology and Infection Volume 8, Issue 9, Pages 579-588 (September 2002) DOI: 10.1046/j.1469-0691.2002.00391.x Copyright © 2002 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Repetitive extragenic palindromic (REP) PCR. Lanes 1 and 13: φX174 DNA/HaeIII digest. Lane 2: PCR control. Lanes 3–12: Amplified DNA segments of strains 11, 41, 223, 568, 584, 660, 1290, 1459, 2294 and 2573, respectively. Clinical Microbiology and Infection 2002 8, 579-588DOI: (10.1046/j.1469-0691.2002.00391.x) Copyright © 2002 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 (a) Isoelectric focusing (pH range 3.5–9.5) of β-lactamases. Lane 1: K. pneumoniae 160 (SHV-5). Lane 2: K. pneumoniae clinical isolate 223. Lane 3: E. coli transconjugant 223–1. Lane 4: K. pneumoniae clinical isolate 1459. Lane 5: E. coli transconjugant 1459-1. Lane 6: K. pneumoniae clinical isolate 2294. Lane 7: E. coli transconjugant 2294-1. The arrow identifies a β-lactamase with a pI of 8.2. (b) Microbiological method of hydrolysis of ceftazidime, cefotaxime, ceftriaxone and aztreonam by β-lactamases. Lane 1: K. pneumoniae 160 (SHV-5). Lane 2: K. pneumoniae clinical isolate 223. Lane 3: E. coli transconjugant 223-1. Lane 4: K. pneumoniae clinical isolate 1459. Lane 5: E. coli transconjugant 1459-1. Lane 6: K. pneumoniae clinical isolate 2294. Lane 7: E. coli transconjugant 2294-1. The arrow identifies the hydrolysis of ceftazidime, cefotaxime, ceftriaxone or aztreonam by the β-lactamase with pI8.2. Clinical Microbiology and Infection 2002 8, 579-588DOI: (10.1046/j.1469-0691.2002.00391.x) Copyright © 2002 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 EcoRI restriction patterns of the transferred plasmid isolated from E. coli transconjugants. Lane 1: λDNA/HindIII digest. Lanes 2–11: EcoRI digests of plasmid isolated from strains 1290-1, 2294-1, 2573-1, 41-1, 223-1, 584-1, 1459-1, 11-1, 568-1, 1201-1. Lane 12: 1-kb DNA ladder. Clinical Microbiology and Infection 2002 8, 579-588DOI: (10.1046/j.1469-0691.2002.00391.x) Copyright © 2002 European Society of Clinical Infectious Diseases Terms and Conditions