The spread of artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion: a molecular epidemiology observational study  Dr Mallika Imwong, PhD, Kanokon Suwannasin, BSc, Chanon Kunasol, MSc, Kreepol Sutawong, MSc, Mayfong Mayxay, MD, Huy Rekol, MD, Frank M Smithuis, MD, Tin Maung Hlaing, MD, Kyaw M Tun, MD, Rob W van der Pluijm, MD, Rupam Tripura, MD, Olivo Miotto, PhD, Didier Menard, PhD, Mehul Dhorda, PhD, Prof Nicholas P J Day, FRCP, Prof Nicholas J White, FRS, Prof Arjen M Dondorp, MD  The Lancet Infectious Diseases  Volume 17, Issue 5, Pages 491-497 (May 2017) DOI: 10.1016/S1473-3099(17)30048-8 Copyright © 2017 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY license Terms and Conditions

Figure 1 Microsatellite haplotypes of the PfKelch13 flanking regions Red box=C580Y. Light blue box=wild type. Each row represents one parasite isolate; white cells indicate identical microsatellite alleles compared with the most frequent allele and dark blue cells indicate differences. The Lancet Infectious Diseases 2017 17, 491-497DOI: (10.1016/S1473-3099(17)30048-8) Copyright © 2017 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY license Terms and Conditions

Figure 2 Study sites A single artemisinin-resistant Plasmodium falciparum PfKelch13 C580Y lineage (K13) has spread over an arc involving Cambodia, Thailand, and Laos. Pfplasmepsin2 amplification (PM2), has emerged subsequently within this lineage. Red dots represent location of the study sites. The Lancet Infectious Diseases 2017 17, 491-497DOI: (10.1016/S1473-3099(17)30048-8) Copyright © 2017 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY license Terms and Conditions