Mercury uptake in Shewanella Oneidensis

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Presentation transcript:

Mercury uptake in Shewanella Oneidensis Alex Szczuka Adviser: Dr. Jeffra Schaefer Prof. Francois Morel Lab

Mercury Cycle Methylmercury = toxic = accumulation

S. Oneidensis: a non-methylator Same environment as methylators Aerobic and anaerobic Is the uptake the same in the presence and lack of oxygen?

Procedure Harvest in exponential growth phase Set up conditions Wash cells Measure mercury content

Mercury uptake is active Uptake with CCCP shown by connected dots;Uptake in the presence of Cysteine disconnected

Uptake could depend on oxygen

Summary Mercury uptake is active Uptake could depend on oxygen

Thank You! Dr. Jeffra Schaefer Prof. Francois Morel PEI

Image references http://www.dec.ny.gov/chemical/8518.html http://web.ornl.gov/info/ornlreview/v37_3_04/article02.shtml