UVA Radiation Impairs Phenotypic and Functional Maturation of Human Dermal Dendritic Cells Laetitia Furio, Odile Berthier-Vergnes, Blandine Ducarre,

Slides:

Advertisements

Similar presentations

In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells Martine A.

Advertisements

Non-small Cell Lung Cancer Induces an Immunosuppressive Phenotype of Dendritic Cells in Tumor Microenvironment by Upregulating B7-H3 Thomas Schneider,

Identification of CD3+CD4−CD8− T Cells as Potential Regulatory Cells in an Experimental Murine Model of Graft-Versus-Host Skin Disease (GVHD) Fumi Miyagawa,

UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin Amine Achachi,

CD90+ Human Dermal Stromal Cells Are Potent Inducers of FoxP3+ Regulatory T Cells Karin Pfisterer, Karoline M. Lipnik, Erhard Hofer, Adelheid Elbe-Bürger

Gianni Gerlini, Hans Peter Hefti, Martin Kleinhans, Brian J

Corticosteroids But Not Pimecrolimus Affect Viability, Maturation and Immune Function of Murine Epidermal Langerhans Cells Wolfram Hoetzenecker, Josef.

Ultraviolet Irradiation Induces Apoptosis in Human Immature, But Not in Skin Mast Cells Sven Guhl, Silke Tapkenhinrichs, Alina Smorodchenko, Andreas Grützkau,

Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells Zia U.A. Mollah, Setsuya Aiba,

Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis Gang Li, Adriana T. Larregina, Robyn.

IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.

PD-1 on Immature and PD-1 Ligands on Migratory Human Langerhans Cells Regulate Antigen-Presenting Cell Activity Victor Peña-Cruz, Sean M. McDonough,

FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis Rahul Purwar, Thomas Werfel, Miriam.

Phosphatidylinositol 3-Kinase/Akt-Dependent and -Independent Protection Against Apoptosis in Normal Human Melanocytes Masahiro Oka, Akiko Kageyama, Mizuho.

Monocyte-Derived Dendritic Cells Formed at the Infection Site Control the Induction of Protective T Helper 1 Responses against Leishmania Beatriz León,

Norito Katoh, Fujiko Soga, Takeshi Nara, Koji Masuda, Saburo Kishimoto

Volume 137, Issue 5, Pages (November 2009)

Human Dermis Harbors Distinct Mesenchymal Stromal Cell Subsets

Non-Coherent Near Infrared Radiation Protects Normal Human Dermal Fibroblasts from Solar Ultraviolet Toxicity Salatiel Menezes Journal of Investigative.

Phenotypic and Functional Outcome of Human Monocytes or Monocyte-Derived Dendritic Cells in a Dermal Equivalent G. Guironnet, C. Dezutter-Dambuyant,

IL-10-Producing Langerhans Cells and Regulatory T Cells Are Responsible for Depressed Contact Hypersensitivity in Grafted Skin Ryutaro Yoshiki, Kenji.

Natural Killer T Cells Are Essential for the Development of Contact Hypersensitivity in BALB/c Mice Chihiro Shimizuhira, Atsushi Otsuka, Tetsuya Honda,

Masato Mizuashi, Tomoyuki Ohtani, Satoshi Nakagawa, Setsuya Aiba

Non-small Cell Lung Cancer Induces an Immunosuppressive Phenotype of Dendritic Cells in Tumor Microenvironment by Upregulating B7-H3 Thomas Schneider,

Cytokine-Induced CEACAM1 Expression on Keratinocytes Is Characteristic for Psoriatic Skin and Contributes to a Prolonged Lifespan of Neutrophils Massilva.

Macrophage Colony-stimulating Factor in Cooperation with Transforming Growth Factor- β1 Induces the Differentiation of CD34+ Hematopoietic Progenitor Cells.

Interleukin-1β But Not Tumor Necrosis Factor is Involved in West Nile Virus-Induced Langerhans Cell Migration from the Skin in C57BL/6 Mice Scott N.

Psoriasis Is Characterized by Accumulation of Immunostimulatory and Th1/Th17 Cell- Polarizing Myeloid Dendritic Cells Lisa C. Zaba, Judilyn Fuentes-Duculan,

Poly(I:C)-Treated Human Langerhans Cells Promote the Differentiation of CD4+ T Cells Producing IFN-γ and IL-10 Laetitia Furio, Hermine Billard, Jenny.

Georg Varga, Nadine Nippe, Sandra Balkow, Thorsten Peters, Martin K

Mycobacterium Tuberculosis-Induced Cell Death of Primary Human Monocytes and Macrophages Is Not Significantly Modulated by Tumor Necrosis Factor-Targeted.

Human Plasmacytoid Dendritic Cells Express Receptors for Anaphylatoxins C3a and C5a and Are Chemoattracted to C3a and C5a Ralf Gutzmer, Brigitta Köther,

Carboxyfullerenes Protect Human Keratinocytes from Ultraviolet-B-Induced Apoptosis Cristiana Fumelli, Alessandra Marconi, Stefano Salvioli, Elisabetta.

The p53-Stabilizing Compound CP Enhances Ultraviolet-B-Induced Apoptosis in a Human Melanoma Cell Line MMRU Yvonne Luu, Gang Li, Dr Journal of.

Impaired Initiation of Contact Hypersensitivity by FTY720

Ekatherina Vassina, Martin Leverkus, Shida Yousefi, Lasse R

PARP Determines the Mode of Cell Death in Skin Fibroblasts, but not Keratinocytes, Exposed to Sulfur Mustard Dana Anderson, Betty Benton, Zhao-Qi Wang,

P38 Mitogen-Activated Protein Kinase Mediates Dual Role of Ultraviolet B Radiation in Induction of Maturation and Apoptosis of Monocyte-Derived Dendritic.

Identification of a Novel CD160+CD4+ T-Lymphocyte Subset in the Skin: A Possible Role for CD160 in Skin Inflammation Sofia Abecassis, Jérôme Giustiniani,

Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity Mayuka Horikawa, Minoru Hasegawa,

Dermal Dendritic Cells Associated with T Lymphocytes in Normal Human Skin Display an Activated Phenotype Alexander D. McLellan, Axel Heiser, Rüdiger.

Different Susceptibility of Malignant versus Nonmalignant Human T Cells Toward Ultraviolet A-1 Radiation-Induced Apoptosis Ritsuko Yamauchi, Akimichi.

Overexpression of IL-4 Alters the Homeostasis in the Skin

B Cells Activated in Lymph Nodes in Response to Ultraviolet Irradiation or by Interleukin-10 Inhibit Dendritic Cell Induction of Immunity Scott N. Byrne,

Volume 117, Issue 3, Pages (September 1999)

Ultraviolet A Irradiation Induces NF-E2-Related Factor 2 Activation in Dermal Fibroblasts: Protective Role in UVA-Induced Apoptosis Ayako Hirota, Yasuhiro.

Yu-Ying He, Jian-Li Huang, Michelle L. Block, Jau-Shyong Hong, Colin F

MHC-Dependent and -Independent Activation of Human Nickel-Specific CD8+ Cytotoxic T Cells from Allergic Donors1 Corinne Moulon, Doris Wild, Hans Ulrich.

Human Beta-Defensin 3 Induces Maturation of Human Langerhans Cell–Like Dendritic Cells: An Antimicrobial Peptide that Functions as an Endogenous Adjuvant

Solar-Simulated Ultraviolet Radiation Induces Abnormal Maturation and Defective Chemotaxis of Dendritic Cells María Mittelbrunn, Reyes Tejedor, Hortensia.

Serotonin Activates Human Monocytes and Prevents Apoptosis

IL-4 and IL-13 Alter Plasmacytoid Dendritic Cell Responsiveness to CpG DNA and Herpes Simplex Virus-1 Jurjen Tel, Ruurd Torensma, Carl G. Figdor, I.

Double-Stranded RNA-Exposed Human Keratinocytes Promote Th1 Responses by Inducing a Type-1 Polarized Phenotype in Dendritic Cells: Role of Keratinocyte-Derived.

Human Langerhans Cells Are More Efficient Than CD14−CD1c+ Dermal Dendritic Cells at Priming Naive CD4+ T Cells Laetitia Furio, Isabelle Briotet, Alexandra.

Dawn Cooper, Jonathan Hales, Richard Camp

Tumor Necrosis Factor-α- and IL-4-Independent Development of Langerhans Cell-Like Dendritic Cells from M-CSF-Conditioned Precursors Jean-Baptiste Barbaroux,

Tomoyuki Ohtani, Setsuya Aiba, Masato Mizuashi, Zia U. A

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Marta E. Polak, Louise Newell, Vadim Y

Plasmacytoid Dendritic Cells: A New Cutaneous Dendritic Cell Subset with Distinct Role in Inflammatory Skin Diseases Andreas Wollenberg, Sandra Günther,

SOCS1 Prevents Potentially Skin-Reactive Cytotoxic T Lymphocytes from Gaining the Ability to Cause Inflammatory Lesions Galaxia Maria Rodriguez, Dante.

Keratinocyte Apoptosis Induced by Ultraviolet B Radiation and CD95 Ligation – Differential Protection through Epidermal Growth Factor Receptor Activation.

Galectin-3 Protects Keratinocytes from UVB-Induced Apoptosis by Enhancing AKT Activation and Suppressing ERK Activation Jun Saegusa, Daniel K. Hsu, Wei.

Machteld M. Tiemessen, Tracey J. Mitchell, Lisa Hendry, Sean J

CD40 Ligation Alters the Cell Cycle of Differentiating Keratinocytes

Patrizia Stoitzner, Christoph H

Volume 31, Issue 5, Pages (November 2009)

GADD45 Regulates G2/M Arrest, DNA Repair, and Cell Death in Keratinocytes Following Ultraviolet Exposure Tomoko Maeda, Atef N. Hanna, Adrian B. Sim,

Generation of Functional Thymocytes in the Human Adult

Presentation transcript:

UVA Radiation Impairs Phenotypic and Functional Maturation of Human Dermal Dendritic Cells Laetitia Furio, Odile Berthier-Vergnes, Blandine Ducarre, Daniel Schmitt, Josette Peguet-Navarro Journal of Investigative Dermatology Volume 125, Issue 5, Pages 1032-1038 (November 2005) DOI: 10.1111/j.0022-202X.2005.23904.x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Phenotypic profile of dermal migrating cells. After 48 h incubation of human dermis in serum-free medium, migrating cells were recovered and processed for flow cytometry. (A) Forward and side scatter identification of the different cell subsets, including dermal dendritic cells (within the gate). (B) Double staining of dermal cells with either anti-CD1c or anti-HLA-DR and the indicated monoclonal antibodies. The total dermal cell suspension was analyzed without any gating. The quadrant gates were set up according to the negative control Ig. The results are representative of at least seven experiments carried out with different donors. Journal of Investigative Dermatology 2005 125, 1032-1038DOI: (10.1111/j.0022-202X.2005.23904.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Ultraviolet A (UVA) irradiation promotes the apoptotic death of human dermal dendrtic cell (DDC). Human dermis was irradiated, or not, with UVA at 2 or 12 J per cm2 and incubated for 48 h in serum-free medium. Migrating cells were then recovered and analyzed for apoptosis using annexin V and propidium iodide staining. (A) DDC were analyzed by flow cytometry, after gating on FSC/SSC parameters according to Figure 1a. Apoptotic cells are located in the lower right quadrant and necrotic cells in the upper right quadrant. Numbers represent the percentage of cells in each quadrant. (B) Results from four independent experiments showing the mean percentage±SD of apoptotic and necrotic cells, as defined in A. *Results statistically different (p<0.005) from the non-irradiated control. Journal of Investigative Dermatology 2005 125, 1032-1038DOI: (10.1111/j.0022-202X.2005.23904.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Phenotypic profiles of resident versus migrating human dermal dendrtic cell (DDC). (A) Human dermis was digested for 2 h with an enzymatic cocktail. The resident dermal cells were recovered and double stained with anti-CD1c and the indicated monoclonal antibodies (mAb). Cells were analyzed by flow cytometry, without any gating. (B) Activation/maturation status of freshly isolated versus migrating DDC. Dermal cells were recovered either after dermis digestion or after a 2-d migration from dermis. Cells were double stained with anti-CD1c and the indicated mAb and analyzed by flow cytometry. Journal of Investigative Dermatology 2005 125, 1032-1038DOI: (10.1111/j.0022-202X.2005.23904.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Ultraviolet A (UVA) alters the allostimulatory function of human dermal dendrtic cell. Human dermis was irradiated, or not, with UVA at 2 or 12 J per cm2 and incubated for 48 h in serum-free medium. Migrating cells were then recovered, and graded number of viable cells was added to purified allogeneic T cells. After 5 d, T cell proliferation was assessed by the addition of 3H-thymidine for 18 h. Results are the mean±SD of triplicate wells and representative of four experiments. The cpm of T cells alone never exceeded 50. The stimulator cell numbers correspond to either total migratory dermal cells (A) or was corrected according to the percentage of viable CD1c+ cells in the suspensions, as assessed by flow cytometry analysis (B). Journal of Investigative Dermatology 2005 125, 1032-1038DOI: (10.1111/j.0022-202X.2005.23904.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions