Rebecca J. Seward, Kevin J. Towner Clinical Microbiology and Infection

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Detection of integrons in worldwide nosocomial isolates of Acinetobacter spp. Rebecca J. Seward, Kevin J. Towner Clinical Microbiology and Infection Volume 5, Issue 6, Pages 308-318 (June 1999) DOI: 10.1111/j.1469-0691.1999.tb00149.x Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Scheme for PCR detection and mapping of class 1 and 2 integron structures. (A) Primers 5′CS and 3′CS are specific to the 5’ and 3’ conserved segments of class 1 integrons, respectively. For PCR mapping the 5′CS primer and primers located at the extremities of possible inserted resistance genes (Table 2) were used. Primer L2 was used additionally to detect ‘empty’ integrons. (B) Primers INT72 and INT74 were used to detect the dhfrIa gene in the class 2 Tn7-type integron structure. INT74 and INT75 were used to amplify the complete Tn7 structure. Clinical Microbiology and Infection 1999 5, 308-318DOI: (10.1111/j.1469-0691.1999.tb00149.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Class 1 integrons detected by PCR in isolates of Acinetobacter spp. (A) Examples of the different integron size type (a–f) obtained. Lane M, 100-bp DNA ladder (Promega). (B) HinfI digestion fingerprints of PCR products belonging to the different size types (a–f). Lane M, 100-bp DNA ladder (Promega). Clinical Microbiology and Infection 1999 5, 308-318DOI: (10.1111/j.1469-0691.1999.tb00149.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 Predicted structures for type I integrons found in Acinetobacter spp. (A) Type a integron. (B) Type b integron. (C) Type d integron. (D) Type e integron. (E) Type f integron. Sizes shown with arrowheads are those detected by PCR analysis of the integron structures (Table 3). Other sizes shown are taken from the literature. Clinical Microbiology and Infection 1999 5, 308-318DOI: (10.1111/j.1469-0691.1999.tb00149.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions