Figure 1. Profiles of the top 50 mRNA probes of the first gene signature identified in the South African cohort. ... Figure 1. Profiles of the top 50 mRNA.

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Figure 1. Profiles of the top 50 mRNA probes of the first gene signature identified in the South African cohort. ... Figure 1. Profiles of the top 50 mRNA probes of the first gene signature identified in the South African cohort. Expression ratios for the top 50 mRNA probes of the first gene signature (HR<sup>+</sup> HPV and CIN1/2/3 gene signature) able to differentiate group C from groups A and B. The first gene signature consisted of 272 mRNA probes with FDR <15% and fold change >1.5 in the comparison of group A + B with group C and P < 0.05 in the comparisons of groups A, B and A + B with group C. Shown are the top 25 mRNA probes that displayed <−1.66-fold change and the top 25 mRNA probes that displayed >1.98-fold change in expression ratios. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Carcinogenesis, Volume 40, Issue 2, 26 October 2018, Pages 225–233, https://doi.org/10.1093/carcin/bgy149 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2. Profiles of the 22 mRNA probes of the second gene signature identified in the South African cohort. ... Figure 2. Profiles of the 22 mRNA probes of the second gene signature identified in the South African cohort. Expression ratios for the 22 mRNA probes of the second gene signature (HR HPV-free and cervical histopathology-free) able to differentiate group A from groups B and C. The second gene signature consisted of 22 genes not represented in the first gene signature (n = 272 genes), with FDR <15% and fold change >1.5 in the comparison of group A with group C and with P < 0.05 in the comparison of group A with group B. Shown are all 22 genes of the signature. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Carcinogenesis, Volume 40, Issue 2, 26 October 2018, Pages 225–233, https://doi.org/10.1093/carcin/bgy149 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 3. Profiles of the 81 mRNA probes of the third gene signature identified in the South African cohort. Expression ... Figure 3. Profiles of the 81 mRNA probes of the third gene signature identified in the South African cohort. Expression ratios for the 81 mRNA probes of the third gene signature (HR<sup>+</sup> HPV and cervical histopathology-free) able to differentiate group B from groups A and C. The third gene signature identified 81 genes not represented in the first gene signature (n = 272 genes), with FDR <15% and fold change >1.5 in the comparison of group B with group C and with P < 0.05 in the comparison of group A with group B. Shown are the top 25 mRNA probes that displayed <−1.66-fold change and the top 24 mRNA probes that displayed >1.98-fold change in expression ratios. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Carcinogenesis, Volume 40, Issue 2, 26 October 2018, Pages 225–233, https://doi.org/10.1093/carcin/bgy149 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 4. Principal component analysis of mRNA probes in the Botswana cohort. Principal component analysis using the 26 ... Figure 4. Principal component analysis of mRNA probes in the Botswana cohort. Principal component analysis using the 26 643 informative mRNA probes [i.e. genes with good detection in all samples (i.e. detection P < 0.05 in all samples), >1.2-fold change between any two samples and background level of expression about 82]. Data are shown for each patient from group R (i.e. women with post-LEEP disease recurrence/relapse, indicated with black circles) and group NR (i.e. women without post-LEEP disease recurrence/relapse, indicated with white squares) of the Botswana cohort. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Carcinogenesis, Volume 40, Issue 2, 26 October 2018, Pages 225–233, https://doi.org/10.1093/carcin/bgy149 The content of this slide may be subject to copyright: please see the slide notes for details.