Evaluation of the inflammatory response in experimentally induced synovitis in the horse: a comparison of recombinant equine interleukin 1 beta and lipopolysaccharide

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General Information Osteoarthritis and Cartilage

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Evaluation of the inflammatory response in experimentally induced synovitis in the horse: a comparison of recombinant equine interleukin 1 beta and lipopolysaccharide T.N. Ross, J.D. Kisiday, T. Hess, C.W. McIlwraith Osteoarthritis and Cartilage Volume 20, Issue 12, Pages 1583-1590 (December 2012) DOI: 10.1016/j.joca.2012.08.008 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Clinical response to intra-articular injection of reIL-1β (100 ng), LPS (0.5 ng) or PBS on lameness grade. Columns lacking common letters indicate significant differences across both treatments (reIL-1β, LPS) and time points (P < 0.0001). *Indicates time point when arthroscopic biopsy was performed. Data are displayed as means ± 95% confidence intervals. Osteoarthritis and Cartilage 2012 20, 1583-1590DOI: (10.1016/j.joca.2012.08.008) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Cytology evaluation of synovial response to intra-articular injection of reIL-1β (100 ng), LPS (0.5 ng) or PBS. For each outcome, columns lacking common letters indicate significant differences across treatments (reIL-1β, LPS), joints (treated, control), and time points. (A) WBCs (P < 0.01). (B) Neutrophils (P < 0.01). Neutrophil values at baseline differed among groups; therefore data were adjusted with PIH 0 values as covariates. (C) Total protein (P < 0.0001). *Indicates time point when arthroscopic biopsy was performed. Data are displayed as means ± 95% confidence intervals. Osteoarthritis and Cartilage 2012 20, 1583-1590DOI: (10.1016/j.joca.2012.08.008) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Synovial fluid response to intra-articular injection of reIL-1β (100 ng), LPS (0.5 ng) or PBS. (A) PGE2 concentration: columns lacking common letters indicate significant differences across treatments (reIL-1β, LPS), joints (treated, control), and time points (P < 0.01). (B) General MMP activity: columns lacking common letters indicate significant differences across joints (treated, control), and time points (P < 0.0001). (C) Glycoaminoglycan concentration: columns lacking common letters indicate significant differences across time points (P < 0.0001); significant difference over time across joints (P = 0.051). *Indicates time point when arthroscopic biopsy was performed. Data are displayed as means ± 95% confidence intervals. Osteoarthritis and Cartilage 2012 20, 1583-1590DOI: (10.1016/j.joca.2012.08.008) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Effect of single intra-articular injection of reIL-1β (100 ng), LPS (0.5 ng) or PBS on synovium gene expression at 8 h post-injection. Values expressed as fold difference relative to LPS controls. (A) MMP-1 expression: columns lacking common letters indicate a significant difference between joints (P = 0.011). (B) ADAMTS-4 expression: columns lacking common letters indicate a significant difference between joints (P < 0.0001). (C) IL-1β gene expression: columns lacking common letters indicate significant difference between joints (P = 0.003) and treatments (P = 0.038). Data are displayed as means ± 95% confidence intervals. Osteoarthritis and Cartilage 2012 20, 1583-1590DOI: (10.1016/j.joca.2012.08.008) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Effect of single intra-articular injection of reIL-1β (100 ng), LPS (0.5 ng) or PBS (controls) on cartilage gene expression at 8 h post-injection. Values expressed as fold difference relative to LPS controls. (A) MMP-1: columns lacking common letters indicate a significant difference between joints (P = 0.002). (B) ADAMTS-4: columns lacking common letters indicate significant difference between joints (P < 0.0001) and treatments (P = 0.054). (C) ADAMTS-5: columns lacking common letters indicate significant difference between joints (P = 0.002). Data are displayed as means ± 95% confidence intervals. Osteoarthritis and Cartilage 2012 20, 1583-1590DOI: (10.1016/j.joca.2012.08.008) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions