Volume 65, Issue 2, Pages 564-574 (February 2004) Acute renal hemodynamic effects of dimanganese decacarbonyl and cobalt protoporphyrin  Begoña Arregui, Bernardo López, Miguel García Salom, Fernando Valero, Concepción Navarro, Francisco J. Fenoy  Kidney International  Volume 65, Issue 2, Pages 564-574 (February 2004) DOI: 10.1111/j.1523-1755.2004.00409.x Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 1 Top: Effects of Co(III)Protoprophyrin IX (CoPP) and Sn Protoporphyrin IX (SnPP) on heme oxygenase (HO) activity in renal homogenates. Middle: Effect of an intrarenal infusion of the CO donor Mn2(CO)10 on blood carboxyhemoglobin concentration (Carboxy Hb) and urinary cGMP excretion. *Indicates significant difference from control. Bottom: Effects of an intravenous infusion of CoPP on plasma bilirubin concentration. *Indicates significant difference from control. Kidney International 2004 65, 564-574DOI: (10.1111/j.1523-1755.2004.00409.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 2 Effects of Co(III)Protoporphyrin IX (CoPP, 16, 80, 160, 800, and 1600nmol · kg-1· min-1, experimental periods E1-E5), Mn2(CO)10 (80nmol · kg-1· min-1 during five consecutive 30-minute periods, from E1 to E5), or CoPP (same doses) + Mn2(CO)10 (80nmol · kg-1· min-1) on renal blood flow (RBF), glomerular filtration rate (GFR), urine flow (UV) and sodium excretion (UNaV).§Indicates a significant difference between the corresponding values in the CoPP and the CoPP + Mn2(CO)10 groups; †indicates significant difference from the corresponding value in the time control group. Kidney International 2004 65, 564-574DOI: (10.1111/j.1523-1755.2004.00409.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 3 Effects of Co(III)Protoporphyrin IX (CoPP, 16, 80, 160, 800, and 1600nmol · kg-1· min-1, experimental periods E1-E5) or Mn2(CO)10 (80nmol · kg-1· min-1 during five consecutive 30-minute periods, from E1 to E5) on cGMP urinary excretion.†Indicates significant difference from the corresponding value in the time control group. Kidney International 2004 65, 564-574DOI: (10.1111/j.1523-1755.2004.00409.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 4 Effects of Co(III)Protoporphyrin IX (CoPP, 16, 80, 160, 800, and 1600nmol · kg-1· min-1, experimental periods E1-E5) or Sn Protoporphyrin IX (SnPP, same doses) on renal blood flow (RBF).*Indicates significant difference from the basal period of the same group. Kidney International 2004 65, 564-574DOI: (10.1111/j.1523-1755.2004.00409.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 5 Top: Effects of CoPP (160 and 800nmol · kg-1· min-1) or NAME (160nmol · kg-1· min-1) on renal cortical NO concentration.*Indicates a significant difference from the basal period; ‡indicates a significant difference from the immediately preceding period. Middle: Effects of Mn2(CO)10 (80nmol · kg-1· min-1), CoPP (800nmol · kg-1· min-1) + Mn2(CO)10 (80nmol · kg-1· min-1), or NAME (160nmol · kg-1· min-1) on renal cortical NO concentration. *Indicates a significant difference from the basal period; ‡indicates a significant difference from the Mn2(CO)10+ CoPP period. Bottom: Effects of Co(III)Protoporphyrin IX (CoPP, 16, 80, 160, 800, and 1600nmol · kg-1· min-1, experimental periods E1-E5), Mn2(CO)10 (80nmol · kg-1· min-1 during five consecutive 30-minute periods, from E1 to E5), or CoPP (same doses) + Mn2(CO)10 (80nmol · kg-1· min-1) on renal NOx (nitrates+nitrites) excretion (U.NOx). §Indicates a significant difference between the corresponding values in the CoPP and the CoPP+Mn2(CO)10 groups; †indicates significant difference from the corresponding value in the time control group. Kidney International 2004 65, 564-574DOI: (10.1111/j.1523-1755.2004.00409.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 6 Top: Effects of Co(III)Protoporphyrin IX (CoPP160: 160nmol · kg-1· min-1, CoPP800: 800nmol · kg-1· min-1) on renal blood flow (RBF) and renal NO concentration (Renal [NO]). Middle: Renal and rectal temperatures during the intrarenal infusion of Co(III)Protoporphyrin IX (same doses). Bottom: Correlation between RBF and Renal [NO] during control conditions and also during the administration of Co(III)Protoporphyrin IX (160 and 800nmol · kg-1· min-1). The data points used are the same depicted in the top graph. Kidney International 2004 65, 564-574DOI: (10.1111/j.1523-1755.2004.00409.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 7 Effects of Co(III)Protoporphyrin IX (CoPP, 80, 160, and 800nmol · kg-1· min-1), or l-NAME (37nmol · kg-1· min-1 for 30 minutes followed by 0.37nmol · kg-1· min-1 throughout the experiment) + CoPP (same doses) on renal blood flow (RBF), glomerular filtration rate (GFR), urine flow (UV), and sodium excretion (UNaV). The absolute values for the basal periods correspond to the values in columns 4, 6, 8, and 10 of Table 1. *Indicates a significant difference from the basal period of the same group; §indicates a significant difference between the corresponding values in the CoPP and the l-NAME + CoPP groups. Kidney International 2004 65, 564-574DOI: (10.1111/j.1523-1755.2004.00409.x) Copyright © 2004 International Society of Nephrology Terms and Conditions