MicroRNAs Dysregulation in Human Malignant Pleural Mesothelioma

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MicroRNAs Dysregulation in Human Malignant Pleural Mesothelioma Veronica Balatti, PhD, Stefania Maniero, PhD, Manuela Ferracin, PhD, Angelo Veronese, PhD, Massimo Negrini, PhD, Gloria Ferrocci, MD, Fernanda Martini, PhD, Mauro G. Tognon, PhD Journal of Thoracic Oncology Volume 6, Issue 5, Pages 844-851 (May 2011) DOI: 10.1097/JTO.0b013e31820db125 Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 1 Cluster analysis of microRNA (miRNA) expression profiles in human normal pleural mesothelial short-term cell culture (HMC) and malignant pleura mesothelioma (MPM) cells. Cluster analysis obtained using the 22 miRNAs described in Table 1, which exhibit at least a twofold differential expression between MPM and HCM samples, and which a p value <0.05 was applied to. The red color represents normal samples, whereas yellow represents tumors. Journal of Thoracic Oncology 2011 6, 844-851DOI: (10.1097/JTO.0b013e31820db125) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 2 Expression of selected microRNAs (miRNAs) in malignant pleura mesothelioma (MPM) samples detected by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR) for microarray data validation. The miRNA was determined by RT-qPCR assay in MPM samples. Data are expressed as a relative fold change over the value of normal controls. Relative expression was calculated using the ΔΔCt method, and the values are expressed as 2−ΔΔCt. As a normalization reference, U6 RNA was used. Journal of Thoracic Oncology 2011 6, 844-851DOI: (10.1097/JTO.0b013e31820db125) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 3 Western Blot analysis of p21 protein. p21 expression in human normal pleural mesothelial short-term cell culture and malignant pleura mesothelioma samples. GAPDH was used as a reference gene. TP53wild-type SKW6.4 cell line, treated with Nutlin-3 for 24 hours, was used as a WB-positive control for p21, whereas the HeLa cell line was used for GAPDH. The numbers below the figure represent the CDKN1A/p21 expression values obtained by microarray analysis (log2 transformed) and refer to the same samples. Journal of Thoracic Oncology 2011 6, 844-851DOI: (10.1097/JTO.0b013e31820db125) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions