HER-2/neu mRNA detection by gene expression profiling

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HER-2/neu mRNA detection by gene expression profiling HER-2/neu mRNA detection by gene expression profiling.A, cluster analysis of transcriptional profile of a series of primary stages I-III invasive breast cancers that were sampled by fine needle aspiration (FNA) biopsy prior to the commencement of neoadjuvant chemotherapy. HER-2/neu mRNA detection by gene expression profiling.A, cluster analysis of transcriptional profile of a series of primary stages I-III invasive breast cancers that were sampled by fine needle aspiration (FNA) biopsy prior to the commencement of neoadjuvant chemotherapy. After mRNA extraction, mRNA expression profiling was performed using the Affymetrix u133 GeneChip™ oligonucleotide microarray system (Affymetrix, Santa Clara, CA). Heat map demonstrates a series of six genes at chromosomal locus 17q12 that were co-amplified with HER-2/neu in a significant number of cases. B, comparison of HER-2/neu mRNA expression levels measured by transcriptional profiling on 32 FNA samples and HER-2/neu gene amplification status determined by FISH on the corresponding core biopsy specimen. Note the close correlation of gene expression with amplification status. (Original data from Pusztai et al., Ref. 114). C, hierarchical clustering after transcriptional profiling of 26 infiltrating breast cancer specimens sampled by FNA using a 62-gene model (25 genes for determining ER status and 37 genes for determining HER-2/neu status). Note the clustering of the HER-2/neu-positive cases and their separation from the HER-2/neu-negative cases. The HER-2/neu-negative ER-positive and HER-2/neu-negative ER-negative tumors can also be separated by this technique. (Original data from Pusztai et al., Ref. 114.)‏ Jeffrey S. Ross et al. Mol Cell Proteomics 2004;3:379-398 © 2004 The American Society for Biochemistry and Molecular Biology