Abnormal retention of nuclear lamina and disorganization of chromatin-related proteins in spermatozoa from DPY19L2-deleted globozoospermic patients Marine.

Slides:

Advertisements

Similar presentations

Clinical benefit using sperm hyaluronic acid binding technique in ICSI cycles: a systematic review and meta-analysis Ronit Beck-Fruchter, Eliezer Shalev,

Advertisements

In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid Federica Riva, Claudia Omes, Roberto Bassani,

Identification and validation of candidate biomarkers involved in human ovarian autoimmunity Purvi V. Mande, Firuza R. Parikh, Indira Hinduja, Kusum.

Kam-Hei So, Cheuk-Lun Lee, William S.B. Yeung, Kai-Fai Lee

Meiotic recombination, synapsis, meiotic inactivation and sperm aneuploidy in a chromosome 1 inversion carrier Gordon Kirkpatrick, Victor Chow, Sai Ma

Chromosomal complement and clinical relevance of multinucleated embryos in PGD and PGS cycles Ahmet Yilmaz, Li Zhang, Xiao Yun Zhang, Weon-Young Son,

Identification and validation of candidate biomarkers involved in human ovarian autoimmunity Purvi V. Mande, Firuza R. Parikh, Indira Hinduja, Kusum.

Attitudes and policies regarding access to fertility care and assisted reproductive technologies in Israel Daniel Sperling, Yael Simon Reproductive.

Cell fate in the early mouse embryo: sorting out the influence of developmental history on lineage choice Samantha A. Morris Reproductive BioMedicine.

P. Bizet, J. Saias-Magnan, E. Jouve, J. M. Grillo, G. Karsenty, C

Sperm macrocephaly syndrome in a patient without AURKC mutations and with a history of recurrent miscarriage Emanuela Molinari, Marzia Mirabelli, Stefania.

In-vitro effects of Thymus munbyanus essential oil and thymol on human sperm motility and function Amirouche Chikhoune, Laurence Stouvenel, Mokrane Iguer-Ouada,

L. Aghajanova, L.C. Giudice Reproductive BioMedicine Online

Norbert Gleicher, Andrea Weghofer, David H. Barad

Abortion legislation: exploring perspectives of general practitioners and obstetrics and gynaecology clinicians Anastasia A. Theodosiou, Oliver R. Mitchell

Anastasia A. Theodosiou, Martin H. Johnson

Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure F. Boitrelle,

Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors Fenju Qin, Jie Zhang, Linsen Zan, Weiqiang.

DNA double strand breaks in human spermatozoa can be predictive for assisted reproductive outcome Andrea Garolla, Ilaria Cosci, Alessandro Bertoldo,

Decreased expression of Wiskott–Aldrich syndrome protein family verprolin-homologous protein 2 may be involved in the development of pre-eclampsia Juan.

Clinical benefit using sperm hyaluronic acid binding technique in ICSI cycles: a systematic review and meta-analysis Ronit Beck-Fruchter, Eliezer Shalev,

An Bracke, Kris Peeters, Usha Punjabi, David Hoogewijs, Sylvia Dewilde

Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos Chien-Hong Chen, Wei-Fang Chang, Chia-Chia Liu, Hwa-Yun Su, Song-Kun.

Telomere length in human blastocysts

Effect of Hsp27 on early embryonic development in the mouse

RBM online: challenges ahead in scientific publishing

Assisted oocyte activation following ICSI fertilization failure

Amir Wiser, Einat Shalom-Paz, Jordana H

‘It's a bit too much fathering this seed’: the meaning-making of the sperm donor in Italian lesbian mother families Vittorio Lingiardi, Nicola Carone,

Role of PAFAH1B1 in human spermatogenesis, fertilization and early embryonic development Gui-Dong Yao, Sen-Lin Shi, Wen-Yan Song, Hai-Xia Jin, Zhao-Feng.

Characterization of lactoferrin receptor on human spermatozoa

Comparative analysis of the seminal plasma proteomes of oligoasthenozoospermic and normozoospermic men Elisa Giacomini, Blendi Ura, Elena Giolo, Stefania.

Effect of Hsp27 on early embryonic development in the mouse

Why are reproductive cancers more common in nulliparous women?

M.M. Francou, J. Ten, R. Bernabeu, J. De Juan

Increased incidence of mosaicism detected by FISH in murine blastocyst cultured in vitro Tanya V. Sabhnani, Aisha Elaimi, Hanan Sultan, Adel Alduraihem,

Ervin Macas, Min Xie, Sara Schaufelberger, Gabriele S

Utility of age-specific serum anti-Müllerian hormone concentrations

What’s on the mind of IVF consumers?

In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid Federica Riva, Claudia Omes, Roberto Bassani,

Evaluation of sperm nuclear integrity in patients with different percentages of decapitated sperm in ejaculates Christine Rondanino, Véronique Duchesne,

S Munné, E Fragouli, P Colls, MG Katz-Jaffe, WB Schoolcraft, D Wells

Biosimilars to recombinant human FSH medicines: comparable efficacy and safety to the original biologic Fernando de Mora, Bart C.J.M. Fauser Reproductive.

Effect of N-acetyl-cysteine after ovarian drilling in clomiphene citrate-resistant PCOS women: a pilot study A. Nasr Reproductive BioMedicine Online

Fertility preservation consultation for women with cancer: are we helping patients make high-quality decisions? Jayeon Kim, Allison M. Deal, Ursula Balthazar,

S. Hendriks, E. A. F. Dancet, A. Meissner, F. van der Veen, M. H

N. Geerts, J. McGrath, J.N. Stronk, T.K. Vanderlick, G. Huszar

Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure F. Boitrelle,

J. M. Feugang, N. Rodriguez-Osorio, A. Kaya, H. Wang, G. Page, G. C

Do endometriomas grow during ovarian stimulation for assisted reproduction? A three- dimensional volume analysis before and after ovarian stimulation

Pim M. W. Janssens, Petra Thorn, Jose A

Vitaly A. Kushnir, Jennifer Choi, Sarah K. Darmon, David F

Cassandra’s prophecy: medic or mother

Aneuploidy is a key causal factor of delays in blastulation: author response to ‘A cautionary note against aneuploidy risk assessment using time-lapse.

The relationship between sperm head retardance using polarized light microscopy and clinical outcomes Belinda G. Vermey, Michael G. Chapman, Simon Cooke,

Donor information considered important to donors, recipients and offspring: an Australian perspective I.S. Rodino, P.J. Burton, K.A. Sanders Reproductive.

Should we be promoting embryo transfer at blastocyst stage?

Biallelic SUN5 Mutations Cause Autosomal-Recessive Acephalic Spermatozoa Syndrome Fuxi Zhu, Fengsong Wang, Xiaoyu Yang, Jingjing Zhang, Huan Wu, Zhou.

Stephen C. Collins, Esther Chan Reproductive BioMedicine Online

Peter Kuckenberg, Caroline Kubaczka, Hubert Schorle

Altered segregation pattern and numerical chromosome abnormalities interrelate in spermatozoa from Robertsonian translocation carriers Anna Godo, Joan.

Pietro Gambadauro, Ramesan Navaratnarajah

Cumulative success rates following mild IVF in unselected infertile patients: a 3-year, single-centre cohort study Daniel Bodri, Satoshi Kawachiya, Michaël.

C Almeida, M Sousa, A Barros Reproductive BioMedicine Online

New approaches to embryo selection

Ovarian reserve screening before contraception?

The nature and origin of binucleate cells in human preimplantation embryos: relevance to placental mesenchymal dysplasia L. Xanthopoulou, J.D.A. Delhanty,

M. Seiringer, M. Maurer, O. Shebl, K. Dreier, G. Tews, S. Ziehr, G

James D. Stanger, Long Vo, John L. Yovich, Ghanim Almahbobi

China’s model of egg donation is a policy lesson for Britain

Presentation transcript:

Abnormal retention of nuclear lamina and disorganization of chromatin-related proteins in spermatozoa from DPY19L2-deleted globozoospermic patients Marine Paci, Razan Elkhatib, Guy Longepied, Sylviane Hennebicq, Julien Bessonat, Blandine Courbière, Patrice Bourgeois, Nicolas Levy, Michael J. Mitchell, Catherine Metzler-Guillemain Reproductive BioMedicine Online Volume 35, Issue 5, Pages 562-570 (November 2017) DOI: 10.1016/j.rbmo.2017.07.013 Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 RT-PCR amplification transcripts for lamin B1, B2, B3, A/C, C2 and for lamin chromatin-partners emerin, Lap2α, BAF and BAF-L from total human spermatozoa RNA. Agarose gel (3%) migration of RT-PCR products amplified from total human RNA from ejaculated globozoospermic spermatozoa for four men (A) and from a fertile patient used as a control (B). Reproductive BioMedicine Online 2017 35, 562-570DOI: (10.1016/j.rbmo.2017.07.013) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 A: Different immunolocalizations of lamin B1 (green) in human globozoospermic spermatozoa relative to the posterior pole labelled using an anti-α-tubulin antibody labelling the sperm tail (red). B: Immunolocalization of lamin B1 (green) in human spermatozoa from a fertile patient with the acrosome identified using lectin PNA (red). Nuclei are counterstained with DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) Reproductive BioMedicine Online 2017 35, 562-570DOI: (10.1016/j.rbmo.2017.07.013) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 A: Percentage of spermatozoa labelled with anti-lamin B1 antibody for the four globozoospermic patients and the fertile control; *% of labelled spermatozoa significantly higher than control (P < 0.05). B: Distribution of the different lamin B1 localizations (posterior pole, periphery or both) on labelled spermatozoa from the four globozoospermic patients (G1–G4) and the fertile control. Reproductive BioMedicine Online 2017 35, 562-570DOI: (10.1016/j.rbmo.2017.07.013) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 A: Different immunolocalizations of SPACA-1 (green) in human globozoospermic spermatozoa. B: Immunolocalization of SPACA-1 in human spermatozoa from the fertile control. Nuclei are counterstained with DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) Reproductive BioMedicine Online 2017 35, 562-570DOI: (10.1016/j.rbmo.2017.07.013) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 Aniline blue staining of spermatozoa: (A) globozoospermic spermatozoa, (B) fertile control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) Reproductive BioMedicine Online 2017 35, 562-570DOI: (10.1016/j.rbmo.2017.07.013) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 6 Immunolocalization of BAF and BAF-L (green) in human spermatozoa from (A) DPY19L2-deleted globozoospermic patients, and (B) fertile patient. BAF and BAF-L signals appear like a small cup at the posterior nuclear pole of ejaculated spermatozoa in the control; this signal was not detectable. Signals are not detectable in globozoospermic spermatozoa. Nuclei are counterstained with DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) Reproductive BioMedicine Online 2017 35, 562-570DOI: (10.1016/j.rbmo.2017.07.013) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions