Hydrosalpinx fluid affects murine embryonic development in a coculture system with epithelial endometrial cells Isabel Carrasco, Elisa Cebral, Ph.D.,

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Hydrosalpinx fluid affects murine embryonic development in a coculture system with epithelial endometrial cells Isabel Carrasco, Elisa Cebral, Ph.D., Ricardo Benitez, M.D., David Vantman, M.D. Fertility and Sterility Volume 75, Issue 5, Pages 1004-1008 (May 2001) DOI: 10.1016/S0015-0282(01)01683-1

Figure 1 Blastocyst development and cell number at 72 hours in an SCS. A total of 91.9% reached the blastocyst stage in the control condition. No significant differences were observed with the presence of different HF concentrations (solid bars). Significant differences were observed in blastocyst cell number (hatched bars) with different HF concentrations (control: 94.2 ± 4.4; 50HF: 70.7 ± 5.3; 70HF: 56.3 ± 5.1, 100HF: 46.8 ± 4.8 cells; ∗P<.0001). Carrasco. Embryotoxic effect of hydrosalpinx. Fertil Steril 2001. Fertility and Sterility 2001 75, 1004-1008DOI: (10.1016/S0015-0282(01)01683-1)

Figure 2 Blastocyst development at 72 hours of coculture. Solid bar represents embryos grown in a normal ECS; hatched bar shows an HECS. A significant difference was observed between ECS and HECS in embryo development: 97.1% and 79.5%, respectively in a control condition (∗∗P<.05). Different HF concentrations were tested. Concentrations over 70% HF caused a decrease in embryonic development: 75.6% and 70.9% with 70HF and 100HF, respectively (∗P<.05), in ECS. No differences were observed in HECS at the HF concentrations tested (82.2%, 83.0%, and 81.8% at 50HF, 70HF, and 100HF, respectively). Carrasco. Embryotoxic effect of hydrosalpinx. Fertil Steril 2001. Fertility and Sterility 2001 75, 1004-1008DOI: (10.1016/S0015-0282(01)01683-1)

Figure 3 Blastocyst cell number in the coculture systems employed. ECS (solid bars) and HECS (hatched bars). Blastocyst cell number in ECS was 90.2 ± 4.9 in the control condition; 65.7 ± 3.9 at 50HF; 47.6 ± 6.3 at 70HF; 39.0 ± 7.5 at 100HF (P<.0001). For blastocyst cells cultured in HECS, the results were 66.6 ± 6.1 in the control condition and 44.6 ± 3.4, 49.5 ± 3.0, and 34.3 ± 2.6 cells at 50HF, 70HF, and 100HF, respectively (∗P<.0001). Carrasco. Embryotoxic effect of hydrosalpinx. Fertil Steril 2001. Fertility and Sterility 2001 75, 1004-1008DOI: (10.1016/S0015-0282(01)01683-1)