A B C p= ns 4T1BR5 GFP expression P10 P20 4T1BR5-Fluc-GFP Count

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A B C p= ns 4T1BR5 GFP expression P10 P20 4T1BR5-Fluc-GFP Count Log fluorescence intensity Supplementary Figure 1: In Vitro characterization of cell line: bioluminescence imaging was performed to assess the functionality of the firefly luciferase gene in engineered 4T1BR5 cells (A). A clonogenic assay was performed to determine differences in the ability of each cell line to form colonies (B). Flow cytometry was used to determine differences in mean GFP fluorescence intensity over ten passages (C).