Influence of pro-inflammatory (IL-1α, IL-6, TNF-α, IFN-γ) and anti-inflammatory (IL-4) cytokines on chondrocyte function A.J Schuerwegh, M.D., Ph.D.,

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Influence of pro-inflammatory (IL-1α, IL-6, TNF-α, IFN-γ) and anti-inflammatory (IL-4) cytokines on chondrocyte function A.J Schuerwegh, M.D., Ph.D., E.J Dombrecht, B.Sc., W.J Stevens, M.D., Ph.D., J.F Van Offel, M.D., C.H Bridts, M.T., L.S De Clerck, M.D., Ph.D. Osteoarthritis and Cartilage Volume 11, Issue 9, Pages 681-687 (September 2003) DOI: 10.1016/S1063-4584(03)00156-0

Fig. 1 Apoptosis, proliferation and NO production of chondrocytes incubated for 48h with different cytokines: with IL-1α (□), TNF-α (○), IFN-γ (▵), IL-4 (▿) or IL-6 (★). Untreated chondrocytes incubated in culture medium without cytokines are represented as 0ng/mL. (a) Percentage of annexin-V positive, PI negative apoptotic cells. (b) Percentage of TUNEL positive apoptotic cells. (c) Proliferation of cells, measured as counts per minute (cpm). (d) μmol/L NO, measured by Griess reaction. Results are expressed as mean (±s.e.m.) of three experiments. ∗P<0.05 vs control chondrocytes. Osteoarthritis and Cartilage 2003 11, 681-687DOI: (10.1016/S1063-4584(03)00156-0)

Fig. 2 MGG staining of chondrocytes cultured in DMEM 10% FCS for 48h without (a) and with 100ng/mL IL-1α (b). Arrows show representative apoptotic chondrocytes with nuclear condensation. Original magnification ×630. Osteoarthritis and Cartilage 2003 11, 681-687DOI: (10.1016/S1063-4584(03)00156-0)

Fig. 3 Apoptosis, proliferation and NO production of chondrocytes incubated for 48h with IL-1α and TNF-α (0.1, 1, 10, 100ng/mL) with (○) and without (□) preincubation with 100ng/mL IL-4. Untreated chondrocytes, incubated in culture medium without cytokines, are represented as 0ng/mL. (a) Percentage of annexin-V positive, PI negative apoptotic cells. (b) Percentage of TUNEL positive apoptotic cells. (c) Proliferation of cells, measured as counts per minute (cpm). (d) μmol/L NO, measured by Griess reaction. Results are expressed as mean (±s.e.m.) of three experiments. ∗P<0.05 vs chondrocytes without preincubation with IL-4. Osteoarthritis and Cartilage 2003 11, 681-687DOI: (10.1016/S1063-4584(03)00156-0)