Intracellular calcium oscillations in articular chondrocytes induced by basic calcium phosphate crystals lead to cartilage degradation C. Nguyen, M.

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Intracellular calcium oscillations in articular chondrocytes induced by basic calcium phosphate crystals lead to cartilage degradation C. Nguyen, M. Lieberherr, C. Bordat, F. Velard, D. Côme, F. Lioté, H.-K. Ea Osteoarthritis and Cartilage Volume 20, Issue 11, Pages 1399-1408 (November 2012) DOI: 10.1016/j.joca.2012.07.017 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Changes in iCa2+ level measured by the Fura-2 method. BACs were treated HA, CA, OCP or MSU crystals or untreated as a control. The values for Rmax and Rmin were calculated by use of 25 μM digitonin, 4 mM EGTA and 60 mM Trisbase to increase the pH >8.3. Osteoarthritis and Cartilage 2012 20, 1399-1408DOI: (10.1016/j.joca.2012.07.017) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 OCP crystal-induced iCa2+ oscillations in isolated BACs. (A) Number of oscillations for single BACs after stimulation with 0.1 mg/mL OCP crystals. Different types of oscillations were observed. Representative results for eight different BACs from the observation field are shown in Refs. 1 and2 (B) Projection in Z plane of an observation field showing all cells in contact with at least one microcrystal (white). Osteoarthritis and Cartilage 2012 20, 1399-1408DOI: (10.1016/j.joca.2012.07.017) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 iCa2+-dependent mechanism of OCP crystal-induced proteoglycan loss. Cultured BCEs similar in size and weight (∼30 mg dry weight each) were treated with or without doses of BAPTA-AM, 0.1 mg/ml OCP, 10 ng/mL rhIL-1β or 1 μM BAPTA-AM alone. Control BCEs were left untreated for 48 h. Proteoglycan loss was assessed by safranin-O staining on paraffin embedded explant sections (representative histologic images of three independent experiments performed in quadruplicate). Osteoarthritis and Cartilage 2012 20, 1399-1408DOI: (10.1016/j.joca.2012.07.017) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions