Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression. Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression. A, Immunoblots for HIF-1α. Human peripheral blood T cells were incubated for 12 h in the absence or presence of 10 μM GF109203X (GX), 100 nM wortmannin (Wort), and immobilized anti-CD3 Ab (CD3). HIF-1α and β-actin proteins were detected in immunoblots as described in Materials and Methods. B, Akt phosphorylation. Human peripheral blood T cells were incubated for 12 h in the absence or presence of immobilized anti-CD3 mAb (CD3) under 21 or 1% oxygen concentration for 12 h. Thr308-phosphorylated form and total Akt protein was detected in immunoblots. Hiroshi Nakamura et al. J Immunol 2005;174:7592-7599 Copyright © 2005 by The American Association of Immunologists