Regional differences in phorbol 12-myristate 13-acetate stimulation of PDGF production in the normal canine aorta John A. van Aalst, MDa, James A. Madura,

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Presentation transcript:

Regional differences in phorbol 12-myristate 13-acetate stimulation of PDGF production in the normal canine aorta John A. van Aalst, MDa, James A. Madura, MDa, Dennis DeLuca, PhDa, Bram R. Kaufman, MDa, David A. Margolin, MDa, Paul L. Fox, PhDb, Linda M. Graham, MDc Journal of Vascular Surgery Volume 32, Issue 3, Pages 584-592 (September 2000) DOI: 10.1067/mva.2000.107761 Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 PMA stimulation of PDGF secretion by proximal and distal canine aorta in organ culture. Aortic segments were incubated with 0, 10, or 100 nmol/L PMA for 72 hours. PDGF was measured by radioreceptor assay (A) ; injury severity of the aortic segments was assessed by scanning electron microscopy (B) and graded on a scale of 0 to 4, 4 being the most severely injured. These experiments were repeated five times through use of aortic segments from five different dogs. Results shown are means of five experiments. Journal of Vascular Surgery 2000 32, 584-592DOI: (10.1067/mva.2000.107761) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 Thrombin stimulation of PDGF secretion by proximal and distal canine aorta in organ culture. Proximal and distal aortic segments from four different dogs were incubated with 0 or 20 U/mL thrombin for 72 hours. PDGF was measured by radioreceptor assay. Results shown are means of four separate experiments. Journal of Vascular Surgery 2000 32, 584-592DOI: (10.1067/mva.2000.107761) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 PMA stimulation of PDGF secretion in cultured canine aortic ECs. Conditioned medium was collected from ECs (n = 6) incubated with 0, 10, or 100 nmol/L PMA for 72 hours. PDGF was measured by radioreceptor assay. Results shown are means of six experiments in which EC cultured from six different dogs were used. Journal of Vascular Surgery 2000 32, 584-592DOI: (10.1067/mva.2000.107761) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 4 PMA stimulation of PDGF secretion in cultured canine aortic SMCs. Conditioned medium was collected from SMCs (n = 6) incubated with 0, 10, or 100 nmol/L PMA for 72 hours. PDGF was measured by radioreceptor assay. Results shown are means of six experiments in which SMCs from six different dogs were used. Journal of Vascular Surgery 2000 32, 584-592DOI: (10.1067/mva.2000.107761) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions

Fig. 5 Protein synthesis in canine proximal and distal aorta in organ culture. Treatment of proximal and distal canine aortic segments with PMA 100 nmol/L (A) and thrombin 20 U/mL (B) for 24 hours did not significantly change protein synthesis. Results shown are means of four separate experiments. Journal of Vascular Surgery 2000 32, 584-592DOI: (10.1067/mva.2000.107761) Copyright © 2000 Society for Vascular Surgery and The American Association for Vascular Surgery, a Chapter of the International Society for Cardiovascular Surgery Terms and Conditions