Polarization microscopy imaging for the identification of unfertilized oocytes after short- term insemination  Yi Guo, M.D., Wenqiang Liu, Ph.D., Yu Wang, M.D., Jiaping Pan, M.D., Shanshan Liang, M.D., Jingling Ruan, M.D., Xiaoming Teng, M.D.  Fertility and Sterility  Volume 108, Issue 1, Pages 78-83 (July 2017) DOI: 10.1016/j.fertnstert.2017.05.009 Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Unfertilized oocytes of different polar body (PB) statuses were subjected to dark-field and bright-field spindle imaging. (A) One PB. (C) Pseudo double PBs. (E) Fragmented PB. (B, D, and F) Bright-field images corresponding to A, C, and E, respectively. (G and H) Human oocytes imaged under a normally inverted microscope. (G) Unfertilized oocyte with one PB. (H) Fertilized oocyte with 2PB. Bars = 50 μm. Fertility and Sterility 2017 108, 78-83DOI: (10.1016/j.fertnstert.2017.05.009) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Calculation of the predictive values for different observation time points. The positive predictive values of the 4-hour, 5-hour, and 6-hour time points were 0.62 (204 of 328), 0.84 (204 of 242), and 0.87 (204 of 235), respectively. Statistically significant differences between the 4-hour group and the 5- and 6-hour groups were found (P=.017) whereas there was only a slight difference between the 5- and 6-hour groups. P<.05 was considered statistically significant. Fertility and Sterility 2017 108, 78-83DOI: (10.1016/j.fertnstert.2017.05.009) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions