Chromosomally Clonal T Cells in the Skin, Blood, or Lymph Nodes of Two Sezary Syndrome Patients Express CD45RA, CD45RO, CDw150, and Interleukin-4, but.

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Chromosomally Clonal T Cells in the Skin, Blood, or Lymph Nodes of Two Sezary Syndrome Patients Express CD45RA, CD45RO, CDw150, and Interleukin-4, but no Interleukin-2 or Interferon-γ Leena Karenko, Hanna Nevala, Maria Raatikainen, Annamari Ranki Journal of Investigative Dermatology Volume 116, Issue 1, Pages 188-193 (January 2001) DOI: 10.1046/j.1523-1747.2001.00207.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 The phenotype of clonal malignant cells in CTCL is detectable with a modified FICTION method in blood, skin, and lymph node samples. Chromosome 8 centromere is visualized with green fluorescence (Alexa 488) in all panels except for (g), where it is red (Alexa 495). Chromosome 1 centromere is visualized blue in (a) and (b), but red in (e). Panels (a)-(e), (g)-(i), and (l) show tissues of case 1, and (f), (k), and (m) of case 2.(a) CD3-positive (Texas Red X) clonal blood cell together with a normal CD3-negative cell; (b) similar CD-3-positive phenotype of a clonal cell in the lymph node (L 2-1); (c) CD4-positive (Rhodamine Red X) clonal cells in the skin shown in two focuses. CD45RO-positive (Texas Red X) clonal cells along with a normal CD45RO-negative cell in (d) a lymph node (L 2-1), (e) the blood (CD45RO dark blue, Alexa 350; red blood cells stain light blue), and (f) a skin lesion (Rhodamine Red X) and (g) a dermatopathic lymph node (L 1-1, green FITC). CD45RA-positive (Texas Red X) clonal malignant cells (h) in the blood along with a normal, CD45RA-negative cell and (i) in a lymph node (L 2-1) along with one CD45RA-negative malignant cell and several normal CD45RA-negative cells. (j) CDw150/SLAM-positive (Texas Red X) and negative clonal cells in the blood along with normal cells. (k) Granzyme-B-negative clonal and normal cells in a lymph node (L 1-2) with no morphologic lymphoma involvement; (l) control staining without antigranzyme antibody (L 1-1), and (m) a rare granzyme-B-positive cell (Rhodamine Red X) in a similar lymph node of case 2 (L 1-2). Scale bars: 10 μm. Journal of Investigative Dermatology 2001 116, 188-193DOI: (10.1046/j.1523-1747.2001.00207.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 The majority of chromosomally clonal cells expressed IL-4, and the cells of case 2 expressed IL-10, but the expression of IL-2 was rare and no IFN-γ was expressed by the malignant cells. Chromosome 8 centromere is visualized with green fluorescence (FITC) in all panels except for (h). Panels (a)–(c), (g), and (h) show lymph node tissue of case 1 (L 2-1), and (d)–(f), skin tissue of case 2 (S 1-2, all photographed with color camera). (a) IL-4-positive (red, Alexa 594) clonal cells together with normal IL-4-negative cells; (b) a brightly IL-4-positive clonal cell (red, Alexa 594); (c) control staining without anti-IL-4 antibody (no red signal); (d) IL-10-positive (red, Alexa 594) and negative clonal cells; (e) an IL-2 positive clonal cell (red, Rhodamine Red X) and IL-2-negative clonal cells; (f) a control staining without primary antibody; (g) IFN-γ-negative clonal cells; (h) a rare IFN-γ-positive cell in a plain immunostaining of the same tissue as in (g). Scale bars: 10 μm. Journal of Investigative Dermatology 2001 116, 188-193DOI: (10.1046/j.1523-1747.2001.00207.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions