Volume 105, Issue 8, Pages 1848-1859 (October 2013) Effects of Detergent β-Octylglucoside and Phosphate Salt Solutions on Phase Behavior of Monoolein Mesophases  Daria S. Khvostichenko, Johnathan J.D. Ng, Sarah L. Perry, Monisha Menon, Paul J.A. Kenis  Biophysical Journal  Volume 105, Issue 8, Pages 1848-1859 (October 2013) DOI: 10.1016/j.bpj.2013.09.009 Copyright © 2013 Biophysical Society Terms and Conditions

Figure 1 Lattice parameters of Pn3m mesophases containing MO and Na or K phosphate salt solutions as a function of salt concentration csalt for different overall loadings of the salt solution in the sample waq (40, 50, or 80 wt%). (A and B) Samples were prepared with solutions of (A) KH2PO4 at 25°C or (B) NaH2PO4 at 22.5°C. Error bars correspond to 1 SD (n = 3 or 4). Lines are linear fits to data. Biophysical Journal 2013 105, 1848-1859DOI: (10.1016/j.bpj.2013.09.009) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 2 (A and B) The identity of mesophases containing MO, βOG, and phosphate salt solutions as a function of the weight fraction of salt solution waq at (A) three different βOG/MO ratios and (B) different salt concentrations. K and Na denote KH2PO4 and NaH2PO4, respectively. Mesophases are denoted as Pn3m (circles), Ia3d (crosses), and Lα (squares). Superimposed symbols at a given composition indicate coexisting phases. The solid curves indicate approximate locations of hydration boundaries based on the appearance of samples and are drawn to guide the eye. To see this figure in color, go online. Biophysical Journal 2013 105, 1848-1859DOI: (10.1016/j.bpj.2013.09.009) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 3 Properties of mesophases prepared with MO, βOG, and solutions of KH2PO4 as a function of weight fraction of salt solution waq at 25°C. (A1–A3) Each chart shows a set of samples formulated with a salt solution of a given concentration csalt at three different βOG/MO ratios. (B1–B3) Each chart shows a set of samples with a constant βOG/MO ratio at three different salt solution concentrations csalt. Mesophases are denoted as Pn3m (circles), Ia3d (crosses), and Lα (squares). For Pn3m mesophases, error bars corresponding to 1 SD are shown. Where not visible, the error bars are smaller than the size of the data points. Error bars for Ia3d and Lα mesophases were consistently smaller than the size of the data points. To see this figure in color, go online. Biophysical Journal 2013 105, 1848-1859DOI: (10.1016/j.bpj.2013.09.009) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 4 Effect of temperature of the properties of mesophases prepared with MO, βOG, and 1.3 M solution of NaH2PO4 at different weight fractions of salt solution waq and three different βOG/MO ratios (0.033, 0.066, and 0.099 w/w). Mesophases are denoted as Pn3m (circles), Ia3d (crosses), and Lα (squares). Error bars are explained in the caption to Figure 3. Biophysical Journal 2013 105, 1848-1859DOI: (10.1016/j.bpj.2013.09.009) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 5 Effect of the cation (Na or K) on the lattice parameters of mesophases prepared with MO, βOG, and salt solutions at different weight fractions of salt solution waq and three different βOG/MO ratios (0.033, 0.066, and 0.099 w/w) at 25°C. Solutions of 1.3 M KH2PO4 or 1.3 M NaH2PO4 were used as indicated in the legend. Mesophases are denoted as Pn3m (circles), Ia3d (crosses), and Lα (squares). Error bars are explained in the caption to Figure 3. Biophysical Journal 2013 105, 1848-1859DOI: (10.1016/j.bpj.2013.09.009) Copyright © 2013 Biophysical Society Terms and Conditions