Recruitment of renal tubular epithelial cells expressing verotoxin-1 (Stx1) receptors in HIV-1 transgenic mice with renal disease Xue-Hui Liu, Clifford.

Slides:

Advertisements

Similar presentations

Volume 77, Issue 9, Pages (May 2010)

Advertisements

Volume 79, Issue 11, Pages (June 2011)

Volume 53, Issue 5, Pages (May 1998)

Volume 62, Issue 6, Pages (December 2002)

Volume 56, Issue 3, Pages (September 1999)

Volume 63, Issue 6, Pages (June 2003)

Volume 67, Issue 2, Pages (February 2005)

Volume 61, Issue 2, Pages (February 2002)

Ana Maria Cuervo, Heinz Hildebrand, Ernst M. Bomhard, J. Fred Dice

The Case ∣ Acute renal failure and anemia

Volume 71, Issue 3, Pages (February 2007)

Volume 58, Issue 5, Pages (November 2000)

Volume 57, Issue 2, Pages (October 2000)

Volume 58, Issue 1, Pages (July 2000)

Volume 69, Issue 12, Pages (June 2006)

Volume 61, Issue 2, (February 2002)

Volume 60, Issue 2, Pages (August 2001)

Expression of somatostatin in the adult and developing mouse kidney

Istvan Arany, Judit K. Megyesi, Jane E.B. Reusch, Robert L. Safirstein

Carlton M. Bates, Heather Kegg, Christina Petrevski, Sandy Grady

Volume 69, Issue 10, Pages (May 2006)

Volume 61, Issue 3, Pages (March 2002)

Cytotoxic effect of Shiga toxin-1 on human proximal tubule cells1

Localization of the prostacyclin receptor in human kidney

Volume 53, Issue 6, Pages (June 1998)

Volume 75, Issue 11, Pages (June 2009)

Kameswaran Surendran, Theodore C. Simon, Helen Liapis, John K. McGuire

Fenghua Zeng, Tomoki Miyazawa, Lance A. Kloepfer, Raymond C. Harris

Volume 59, Issue 3, Pages (March 2001)

Volume 62, Issue 3, Pages (September 2002)

Calcium oxalate crystal localization and osteopontin immunostaining in genetic hypercalciuric stone-forming rats Andrew P. Evan, Sharon B. Bledsoe, Susan.

Volume 55, Issue 4, Pages (April 1999)

Volume 77, Issue 9, Pages (May 2010)

Xue-Hui Liu, Achim Aigner, Anton Wellstein, Patricio E. Ray

Detergent-resistant globotriaosyl ceramide may define verotoxin/glomeruli-restricted hemolytic uremic syndrome pathology Fahima Khan, François Proulx,

Volume 76, Issue 2, Pages (July 2009)

M. Svensson, H. Irjala, C. Svanborg, G. Godaly Kidney International

Volume 63, Issue 2, Pages (February 2003)

Alisa K. Hughes, Peter K. Stricklett, Doug Schmid, Donald E. Kohan

Volume 72, Issue 10, Pages (November 2007)

Volume 57, Issue 2, Pages (October 2000)

Volume 68, Issue 6, Pages (December 2005)

Gene transfer of truncated IκBα prevents tubulointerstitial injury

Magnus Åbrink, Eric Larsson, Anders Gobl, Lars Hellman

Yongji Wang, Megan L. Borchert, Hector F. DeLuca Kidney International

Volume 54, Issue 2, Pages (August 1998)

Leslie A. Bruggeman, Scott H. Adler, Paul E. Klotman

Xue-Hui Liu, Achim Aigner, Anton Wellstein, Patricio E. Ray

Renal L-type fatty acid-binding protein mediates the bezafibrate reduction of cisplatin- induced acute kidney injury K. Negishi, E. Noiri, R. Maeda, D.

Volume 78, Issue 7, Pages (October 2010)

In situ non-radioactive detection of nuclear factors in paraffin sections by Southwestern histochemistry Miguel Angel Hernández-Presa, Carmen Gómez-Guerrero,

Autoantibodies against intercalated cells in Sjögren's syndrome

Calcitonin receptor isoforms expressed in the developing rat kidney

Volume 74, Issue 6, Pages (September 2008)

Volume 69, Issue 3, Pages (February 2006)

Volume 59, Issue 5, Pages (May 2001)

Volume 80, Issue 5, Pages (September 2011)

Volume 59, Issue 4, Pages (April 2001)

Volume 59, Issue 2, Pages (February 2001)

Volume 54, Issue 5, Pages (November 1998)

Volume 75, Issue 5, Pages (March 2009)

Adenovirus-mediated gene transfer to renal glomeruli in rodents

Volume 76, Issue 1, Pages 6-7 (July 2009)

Mineralocorticoid selectivity: Molecular and cellular aspects

Increased importin α protein expression in diabetic nephropathy

Volume 69, Issue 10, Pages (May 2006)

Volume 75, Issue 3, Pages (February 2009)

Volume 55, Issue 2, Pages (February 1999)

Is complement a target for therapy in renal disease?

Volume 70, Issue 9, Pages (November 2006)

Presentation transcript:

Recruitment of renal tubular epithelial cells expressing verotoxin-1 (Stx1) receptors in HIV-1 transgenic mice with renal disease Xue-Hui Liu, Clifford A. Lingwood, Patricio E. Ray Kidney International Volume 55, Issue 2, Pages 554-561 (February 1999) DOI: 10.1046/j.1523-1755.1999.00278.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Verotoxin-1 (Stx1) binding and immunohistochemistry Gb3 staining in control and HIV transgenic mice. (A–D) Fluorescent staining using FITC-labeled Stx 1 in control (D) and HIV transgenic kidneys (A and C). In control kidneys, Stx1 staining was predominately localized to renal collecting ducts and distal tubules. HIV transgenic mice showed a significant recruitment of Stx1 binding sites in RTEcs. (B) A transgenic renal section pretreated overnight with 1 U/ml α-galactosidase and then incubated with FITC-labeled Stx1, as described in the Methods section. As expected, Stx1 staining was significantly reduced. (E and F) Immunohistochemistry staining with an anti-Gb3 monoclonal antibody in control (F) and HIV transgenic (E) kidneys. Similar staining patterns were found for both anti-Gb3 and Stx1 staining. Note the significant recruitment of RTEcs expressing Gb3 in HIV transgenic mouse kidneys (E). As shown in panels G and H, Gb3 (red) was predominately localized to dilated collecting ducts and distal tubules in HIV-Tg mice with renal disease (costained blue with the lectin peanut agglutinin). (Magnification: A, B, C, and G ×200; D, E, and F ×100, H ×500) Kidney International 1999 55, 554-561DOI: (10.1046/j.1523-1755.1999.00278.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 Detection of Gb3 in control and HIV transgenic kidneys. VT-1 (Stx1) thin layer chromatography (TLC) overlay studies were performed with lipids extracts from control and transgenic diseased kidneys (4mg of tissue from each kidney). Lanes A and B show control kidneys; lanes C and D show transgenic kidneys; lanes E and F show Gb3 and Gb2 standards. The Gb2 standard (upper bands in E and F) is from a marine sponge and does not line up well with the mammalian Gb2 (detected in lane C upper band). Abbreviations are: GC, galactosyl ceramide; LC, lactosylceramide; Gb2, galabiosylceramide; Gb3, globotriaosylceramide; Gb4, globotetraosylceramide. Kidney International 1999 55, 554-561DOI: (10.1046/j.1523-1755.1999.00278.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Kidney International 1999 55, 554-561DOI: (10. 1046/j. 1523-1755. 1999 Kidney International 1999 55, 554-561DOI: (10.1046/j.1523-1755.1999.00278.x) Copyright © 1999 International Society of Nephrology Terms and Conditions