The interaction of lubricin/proteoglycan-4 (PRG4) with toll-like receptor 2: an anti- inflammatory role of PRG4 in synovial fluids from patients with osteoarthritis

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The interaction of lubricin/proteoglycan-4 (PRG4) with toll-like receptor 2: an anti- inflammatory role of PRG4 in synovial fluids from patients with osteoarthritis A. Alquraini, S. Garguilo, L. Zhang, T. Schmidt, G. Jay, K.A. Elsaid Osteoarthritis and Cartilage Volume 24, Pages S499-S500 (April 2016) DOI: 10.1016/j.joca.2016.01.915 Copyright © 2016 Terms and Conditions

Fig. 1 Concentration-dependent binding of rhPRG4 and nhPRG4 to immobilized TLR2. rhPRG4 exhibited a concentration-dependent binding at 50, 10 and 1 μg/ml. nhPRG4 exhibited a concentration-dependent binding at 50 and 10μg/ml. *p < 0.001. Data represents the mean ± S.D. of 4 independent experiments. Dashed line represents non-specific binding (background). Osteoarthritis and Cartilage 2016 24, S499-S500DOI: (10.1016/j.joca.2016.01.915) Copyright © 2016 Terms and Conditions

Fig. 2: Inhibition of Pam3CSK4-induced activation of TLR2 by nhPRG4 (50, 100 and 150 μg/ml).Pam3CSK4 significantly activated TLR2. nhPRG4 significantly reduced Pam3CSK4-induced TLR2 activation. *p < 0.001. Absorbance values across groups were normalized to untreated cells (control). Data represents the mean ± S.D. of 4 independent experiments. Osteoarthritis and Cartilage 2016 24, S499-S500DOI: (10.1016/j.joca.2016.01.915) Copyright © 2016 Terms and Conditions

Fig. 3. Activation of toll-like receptor 2 expressing human embryonic kidney (TLR2-HEK) cells by normal (n = 3) and osteoarthritic (OA; n = 8) synovial fluids (SF). OA SFsignificantly activated TLR2 compared to control and normal SF. nhPRG4 treatment (100μg/ml) significantly reduced OA SF-induced TLR2 activation. *p < 0.001. Absorbance values across groups were normalized to untreated cells (control). Data represents the mean ± S.D of 3 independent experiments. Osteoarthritis and Cartilage 2016 24, S499-S500DOI: (10.1016/j.joca.2016.01.915) Copyright © 2016 Terms and Conditions