Microscopes.

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Presentation transcript:

Microscopes

Microscopes Eyepiece- contains the 10X magnifying lens Adjustment knob/focus- focuses the image under low power and high power

Microscopes Objective lenses- two or three separate lenses that contain varying powers of magnifying lenses Stage and stage clips- supports and hold the microscope slide in place while viewing Diaphragm- controls the amount of light available

Microscopes Arm- supports the body tube which connects the eyepiece to the set of objective lenses Light source- a mirror, external or internal light source that shines light through the object being viewed Base- supports the microscope

Microscopes When looking through a microscope, the lighted area is the field of view. Adjust the diaphragm until an adequate amount of light is available. To make the field of view brighter, open the diaphragm. To make the field of view darker, close the diaphragm.

Microscopes To view an object under the microscope, first focus on the lowest power objective lens. Then change to the highest power objective lens if necessary. When focusing the image under low power objective, use the coarse adjustment knob. Use only the fine adjustment knob to sharpen the focus when using the high power objective. Scanning Objective Lens: 4x (total magnification= 40x)

Microscopes 4x 40x 10x 100x 400x Magnification Total Magnification (ocular lens=10x) Objective Lenses Elodea leaf magnified Scanning 4x 40x Low Power 10x 100x High Power 400x To calculate the magnification of objects seen through a microscope, multiply the magnification of the eyepiece times the magnification of the objective lens being used. Objects on the slide move in the opposite direction when being viewed through the eyepiece (for example, if the slide is moved to the left, the object being viewed appears to move to the right).