Ralf Jansen, Wojciech Dzwolak, Roland Winter Biophysical Journal

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Presentation transcript:

Amyloidogenic Self-Assembly of Insulin Aggregates Probed by High Resolution Atomic Force Microscopy Ralf Jansen, Wojciech Dzwolak, Roland Winter Biophysical Journal Volume 88, Issue 2, Pages 1344-1353 (February 2005) DOI: 10.1529/biophysj.104.048843 Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 1 The overview of insulin fibrillization at 60° (left) and 70°C (right) monitored by time-lapse AFM. The following images correspond to: 0min (a, j), 1min (b, k), 5min (c, l), 10min (d, m), 20min (e, n), 30min (f, o), 1h (g, p), 1 day (h, q), and 1 week (i, r). The scan size of each picture is 10μm×10μm. Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 2 An AFM height image of fresh, unheated insulin solution, pH 1.6, T=20°C (a). The magnified image in b shows spherical shapes of the protein particles. Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 3 (a) AFM height image of the early insulin aggregates formed during the first 30s of incubation at 60°C; the arrows mark the elongated, prefibrillar loose aggregates. Averaged size-distribution of the particles (b). Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 4 First rodlike insulin aggregates formed after 1min at 60°C (a and b). An example of the measurement of filament height (c). Particles of prefibrillar aggregates tend to cluster in the proximity of the early fibers (b, d, and e)—marked in green line. Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 5 Height (a) and phase-shift (b) AFM images of a cluster of insulin aggregates formed after 5min incubation at 60°C. Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 6 Morphological variability in insulin amyloid samples obtained after 10-min incubation at 70°C (a–c). Fibrils diameters: 4.2±0.20nm (red), 5.5±0.57nm (turquoise), 7.2±0.50nm (blue), and 9.0±0.36nm (yellow). Image of fibers obtained through 10-min incubation of native insulin at 60°C (d). Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 7 AFM images of mature insulin amyloids grown at 70°C after a 2-min (a) or 30-min (b) incubation. Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 8 Generalized scheme of the multipathway fibrillization of insulin. The lateral interaction of early, prefibrillar forms with protofibrils and protofilaments, followed by the lateral association of protofilaments, is a self-assembly route alternative to the hierarchical intertwining of protofilaments. The observed polymorphism of mature amyloid samples suggests that, under the given conditions, insulin fibrillization proceeds from both pathways. Biophysical Journal 2005 88, 1344-1353DOI: (10.1529/biophysj.104.048843) Copyright © 2005 The Biophysical Society Terms and Conditions