Strontium causes osteomalacia in chronic renal failure rats Iris Schrooten, Walter Cabrera, William G. Goodman, Simonne Dauwe, Ludwig V. Lamberts, Rita Marynissen, Walter Dorriné, Marc E. De Broe, Patrick C. D’Haese  Kidney International  Volume 54, Issue 2, Pages 448-456 (August 1998) DOI: 10.1046/j.1523-1755.1998.00035.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Detailed time scheme of the experimental rat study. Abbreviations are: B, blood sampling for creatinine, Al, Sr, Ca, P, and iPTH; U, urine sampling for creatinine, Al, Sr, and Ca. Kidney International 1998 54, 448-456DOI: (10.1046/j.1523-1755.1998.00035.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Goldner staining of a control (a), a Sr-loaded (b) and an Al-loaded rat (c). Note the increased amounts of osteoid in the Sr-loaded animal and the absence of osteoid in the Al-loaded rat. Kidney International 1998 54, 448-456DOI: (10.1046/j.1523-1755.1998.00035.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 3 Histological data of the various groups. *P < 0.05; **P < 0.01; ***P < 0.001. Kidney International 1998 54, 448-456DOI: (10.1046/j.1523-1755.1998.00035.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 4 (left) Histochemical analysis of a histological bone section of a Sr-loaded rat. (a) Rhodizonate staining demonstrates the presence of Sr at the osteoid/calcification front (× 81). (b) Magnification (×202.5) clearly demonstrates the presence of small consecutive lines. (c) Schematic summary of the histochemical result. Kidney International 1998 54, 448-456DOI: (10.1046/j.1523-1755.1998.00035.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 5 (right) Electron probe X-ray microanalysis of a histological bone section of a Sr-loaded rat. (a) Ca (white) is homogeneously present in calcified bone. (b) Sr (yellow) could be demonstrated in calcified bone, with most of the Sr present near the mineralization front. (c) Schematic summary of the EPXMA analysis. Kidney International 1998 54, 448-456DOI: (10.1046/j.1523-1755.1998.00035.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 448-456DOI: (10. 1046/j. 1523-1755. 1998 Kidney International 1998 54, 448-456DOI: (10.1046/j.1523-1755.1998.00035.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 448-456DOI: (10. 1046/j. 1523-1755. 1998 Kidney International 1998 54, 448-456DOI: (10.1046/j.1523-1755.1998.00035.x) Copyright © 1998 International Society of Nephrology Terms and Conditions