Analysis of miR‐1a‐3p target genes in the context of tumor‐induced hypersensitivity in the bone metastatic pain model QRT‐PCR analysis demonstrating induction.

Slides:



Advertisements
Similar presentations
H. influenzae genes differentially regulated during growth in complex media and during lung infection in the presence of corticosteroids AVenn diagram.
Advertisements

The expressions of H19 and Igf1r are significantly decreased in the eutopic endometrium of women with endometriosis compared to those without endometriosis.
Activation of Src Family Kinases in Spinal Microglia Contributes to Formalin-Induced Persistent Pain State Through p38 Pathway  Yong-Hui Tan, Kai Li,
Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.
Statistical analysis of transgene expression in pigs generated by lentiviral gene transfer. Statistical analysis of transgene expression in pigs generated.
Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.
Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice.
Trx80 is generated by α‐secretases. A
PERK activator reduces behavioral deficits and neurodegeneration in vivo PERK activator reduces behavioral deficits and neurodegeneration in vivo A–CYoung.
The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.
TFE3 regulates glucose homeostasis
Generation and characterization of Vps15‐null mice and cells. A
AAV8‐hAAT‐FGF21 treatment improves liver fibrosis
Differential accumulation of substrates in vivo
The absence of COL6 in PyMT mice sensitizes tumours to cisplatin treatment.A.Eleven week old FP635/PyMT and FP635/PyMT/COL6−/− mice were given cisplatin.
Metabolic profile of Tfe3 KO mice fed a HFD
Up‐ or down‐regulation of microRNAs (miRNAs) in sensory neurons of the dorsal root ganglia (DRG) in a model of bone metastases pain A.Increase in frequency.
In vitro and in vivo validation of Clcn3 as a miR‐1a‐3p target gene and its functional contribution to tumor‐induced mechanical hypersensitivity in the.
TGF‐β1 and Sema3A are downregulated in vivo by increasing VEGF doses Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones.
EphB4 regulates VEGF‐induced phosphorylation of endothelial ERK1/2 downstream of VEGF‐R2 activation EphB4 regulates VEGF‐induced phosphorylation of endothelial.
Clinical testing of calcineurin inhibitors cyclosporine A and tacrolimus in autoimmune disorders Clinical testing of calcineurin inhibitors cyclosporine.
No neurodegenerative phenotype observed in single Aph1a and Aph1bc cKO Cre+ mice No neurodegenerative phenotype observed in single Aph1a and Aph1bc cKO.
Effects of GM1 on astroglial and microglial markers
Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),
TFE3 prevents diet‐induced obesity and metabolic syndrome
Identification of miR‐499 targets
Validation of astrocyte and microglial proteomic markers that increase across the ALS‐FTD spectrum Validation of astrocyte and microglial proteomic markers.
Cycling resident cardiomyocytes in the peri‐infarct area. A–C
Combination of OTX‐015 and PD does not induce overt toxicity
PERK activator treatment protects neurite network
LIMD1 expression is regulated by hypoxia
Intra‐eWAT administration of FGF21 vectors in ob/ob mice
Nec‐1 inhibits the phosphorylation and aggregation of tau
Fig. 5 Local gel scaffold for T cell memory response.
Rescuing mGPDH deficiency improves skeletal muscle regeneration during obesity and diabetes Rescuing mGPDH deficiency improves skeletal muscle regeneration.
Impaired ECM protein synthesis in infarcted area of S100a4cre+/− × Fstl1flox/flox mice Impaired ECM protein synthesis in infarcted area of S100a4cre+/−
IR induces cellular damage and a loss of nerves and SOX2 and SOX2+ progenitors can replenish mouse acinar cells in response to cholinergic mimetics IR.
Peptidergic nociceptor markers are reduced in DRG from Nav-Tsc2 mice.
IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.
QRT–PCR analysis of tissue‐specific candidate gene expression in response to reduced IIS qRT–PCR analysis of tissue‐specific candidate gene expression.
LV.IDS and LV.IDS.ApoEII improve brain‐specific IDS activity and express supra‐physiological levels of active IDS in peripheral organs LV.IDS and LV.IDS.ApoEII.
PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program by miR‐499 PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program.
Inhibition of NAMPT using the potent inhibitor FK866 sensitizes additional tumour cells to olaparib.A‐E.Sensitivity of the cell lines MDAMB468 (A), SUM149.
DOK7 gene therapy suppresses motor nerve terminal degeneration at the NMJ in ALS mice DOK7 gene therapy suppresses motor nerve terminal degeneration at.
In vivo efficacy of Olaparib in PTEN deficient xenografts.
Increased LIMD1 expression in hypoxia inhibits tumour growth and vascularisation Increased LIMD1 expression in hypoxia inhibits tumour growth and vascularisation.
Induction of LIMD1 in hypoxia inhibits HIF‐1‐mediated gene expression
GPR120 deficiency alters the expression of genes involved in glucose and lipid metabolism in BAT GPR120 deficiency alters the expression of genes involved.
GPRC5A is hypoxia/HIF‐induced in vivo
miR‐34a‐5p acts in PDGFRα+ cells to block lung alveolarization
Validation of astrocyte and microglial proteomic markers that increase across the ALS‐FTD spectrum Validation of astrocyte and microglial proteomic markers.
MiR‐34a‐5p functionally contributes to arrested lung alveolarization in response to hyperoxia miR‐34a‐5p functionally contributes to arrested lung alveolarization.
Drp1 knockdown prevents mitophagy
Effects of KY‐02327 on OVX‐induced osteoporosis model mice
Rescue of motor neurons from death The ventral root of the fifth lumbar segment (L5). Rescue of motor neurons from death The ventral root of the fifth.
The CHCH domain is necessary for mitochondrial import of CHCHD10
Treatment with AZD5363 reduces the proportion of proliferating vascular endothelial cells and following RT, the influx of CD11b+ bone marrow‐derived cells.
Tissue‐specific differences in the levels of CoQ9, CoQ10, and DMQ9, and in the DMQ9/CoQ9 ratio after β‐RA treatment in Coq9R239X mice Tissue‐specific differences.
Sox2 is essential for SLG regeneration following radiation injury
Antagonizing miR‐34a function partially restores proper lung alveolarization Antagonizing miR‐34a function partially restores proper lung alveolarization.
Effect of D‐Asp treatment on myelination and remyelination in cerebellar organotypic slices Effect of D‐Asp treatment on myelination and remyelination.
Catalytic activity of mTERT is needed for increased healthspan and life‐span extension in AAV9‐mTERT treated mice.A.Scheme of mTERT‐wt and mTERT‐DN, a.
AS aggregates interact with SERCA in SH‐SY5Y cells
P2X4R blockade increases pro‐inflammatory gene expression after EAE
Symptomatic rescue of double‐transgenic mice without decreased protein aggregation The expression of exogenous HMGB1‐FLAG (detected with an anti‐FLAG antibody)
CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair depigmentation CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair.
LIMD1 hypoxic induction occurs via HIF‐1 and facilitates formation of a hypoxic HIF‐1 degradation complex LIMD1 hypoxic induction occurs via HIF‐1 and.
Tangle formation and downregulation of miR‐132‐3p in LOAD A‐G
Niacin suppresses pro‐inflammatory cytokine expression in macrophages in DSS‐induced colitis in mice Niacin suppresses pro‐inflammatory cytokine expression.
MiR-200c suppresses tumor growth and metastasis of CRC in vivo by targeting Sox2. MiR-200c suppresses tumor growth and metastasis of CRC in vivo by targeting.
Presentation transcript:

Analysis of miR‐1a‐3p target genes in the context of tumor‐induced hypersensitivity in the bone metastatic pain model QRT‐PCR analysis demonstrating induction of predicted miR‐1a‐3p target genes in lumbar DRGs in vivo following suppression of miR‐1a‐3p expression via inhibitor delivery intrathecally in vivo. * denotes p <:0.0001 as compared to mismatch‐inhibitor group.QRT‐PCR analysis representing changes in expression of miR‐1a‐3p target genes in ipsilateral lumbar DRGs in vivo at day 8 in the bone metastases model. * denotes p = 0.041 as compared to sham group.Time course of tumor‐induced downregulation of Clcn3 expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.002 on PID‐4, 0.003 on PID‐8, 0.05 on PID‐10 and 0.04 on PID‐15 as compared to sham group.Time course of tumor‐induced changes in miR‐1a‐3p expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.04 on PID‐4, 0.002 on PID‐8, and 0.04 on PID‐10 as compared to sham group.In panels A, B, C &D statistical P value was calculated by ANOVA followed by post hoc Fischer's test, n = 3 mice per group.Immunohistochemical analysis of expression of Clcn3 protein in mouse DRG and colabeling with isolectin‐B4‐binding (IB4) non‐peptidergic nociceptors and peptidergic (CGRP‐positive) nociceptors. Analysis of miR‐1a‐3p target genes in the context of tumor‐induced hypersensitivity in the bone metastatic pain model QRT‐PCR analysis demonstrating induction of predicted miR‐1a‐3p target genes in lumbar DRGs in vivo following suppression of miR‐1a‐3p expression via inhibitor delivery intrathecally in vivo. * denotes p <:0.0001 as compared to mismatch‐inhibitor group.QRT‐PCR analysis representing changes in expression of miR‐1a‐3p target genes in ipsilateral lumbar DRGs in vivo at day 8 in the bone metastases model. * denotes p = 0.041 as compared to sham group.Time course of tumor‐induced downregulation of Clcn3 expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.002 on PID‐4, 0.003 on PID‐8, 0.05 on PID‐10 and 0.04 on PID‐15 as compared to sham group.Time course of tumor‐induced changes in miR‐1a‐3p expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.04 on PID‐4, 0.002 on PID‐8, and 0.04 on PID‐10 as compared to sham group.In panels A, B, C &D statistical P value was calculated by ANOVA followed by post hoc Fischer's test, n = 3 mice per group.Immunohistochemical analysis of expression of Clcn3 protein in mouse DRG and colabeling with isolectin‐B4‐binding (IB4) non‐peptidergic nociceptors and peptidergic (CGRP‐positive) nociceptors. Scale bars represent 50 µm. Kiran Kumar Bali et al. EMBO Mol Med. 2013;5:1740-1758 © as stated in the article, figure or figure legend