qRT-PCR validation of differential expression. qRT-PCR validation of differential expression. The expression profiles of the 14 genes identified by Glasso were examined by qRT-PCR of RNA extracted from euhydrated (control) and dehydrated (DH) 12-week-old male SD SON. Of the original 14 genes in the Glasso network (Fig. 2A) 10 had significantly different relative expression levels. Error bar, SEM; *p < 0.05; **p < 0.01; ***p < 0.001; n = 10 for control and n = 11 for DH. Su-Yi Loh et al. eNeuro 2017;4:ENEURO.0243-17.2017 ©2017 by Society for Neuroscience