T Cell LEGO: Identifying the Master Builders and What They Do Jasmine Li, Stephen J. Turner  Immunity  Volume 48, Issue 2, Pages 185-187 (February 2018) DOI: 10.1016/j.immuni.2018.02.004 Copyright © 2018 Elsevier Inc. Terms and Conditions

Figure 1 T Cell Commitment Is Locked In by the Interplay between “Master” Transcription Factors and the Chromatin Landscape Thymic T cell progenitors are committed to the double-negative (DN) stage of differentiation (DN1, DN2, DN3, DN4) after NOTCH signaling, resulting in TCF-1 upregulation (A). During the DN2-DN3 transition, there is a stepwise and unidirectional change in higher-order chromatin structures where repressive B compartments that contain T cell-lineage-specific genes, such as the master regulator Bcl11b, are switched to transcriptionally active A compartments (B). This compartment switch is coincides with TCF-1 binding to heterochromatin structures that then remodels these structures to have greater chromatin accessibility, increased chromatin contacts localized within specific topologically associating domains (TADs), and transcriptional upregulation (C). The upregulation of BCL11b at this stage then serves to stabilize the intra-TAD contacts and remodel chromatin architecture within mature T cell subsets. Immunity 2018 48, 185-187DOI: (10.1016/j.immuni.2018.02.004) Copyright © 2018 Elsevier Inc. Terms and Conditions