Joseph Murray Mentor: Dr. Ariel Anbar

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Presentation transcript:

Joseph Murray Mentor: Dr. Ariel Anbar Carbon and nitrogen isotopic composition of hot spring biofilms from Yellowstone National Park Joseph Murray Mentor: Dr. Ariel Anbar Title page

Astrobiology at Yellowstone National Park Yellowstone abounds with diverse hydrothermal ecosystems Provides an excellent opportunity to observe many unique organisms living in extreme habitats Unique chance to search for variations in the elemental requirements for life Biological Stoichiometry yellowstone Jeff Havig, Ph.D. Candidate

Isotope fractionation What is a bioflim? H2O 12C 14N 12C 15N Nutrients Biofilms and isotope fractionation Isotope fractionation

Bison Pool 93º C 67º C 50º C Source Flow Direction High Temperature Intro to Bison Pool Low Temperature 93º C 67º C 50º C “Photosynthetic Fringe”

Carbon Cycling In Bison Pool: 2005-2006 This plot shows the D13C (fractionation from DIC) values for biofilms from ‘Bison Pool’ collected in 2005 and 2006. The lines and arrows are put in place only as estimates of averages and to help illustrate the assumed trend in fractionation. Bison Pool C story 2005-2006 (J. Havig, in prep.)

Carbon Cycling In Bison Pool: 2005-2007 Bison Pool w/ 2007 Data 2007 Biofilm (J. Havig, in prep.) This plot shows the D13C (fractionation from DIC) values for biofilms from ‘Bison Pool’ collected in 2005, 2006 and 2007. The lines and arrows are put in place only as estimates of averages and to help illustrate the assumed trend in fractionation.

Carbon Cycling In Bison Pool: 2005-2007 TCA PEP Bison Pool w/ 2007 Data Carbon fixation pathways-supported by metagenomic data 2007 Biofilm (J. Havig, in prep.) This plot shows the D13C (fractionation from DIC) values for biofilms from ‘Bison Pool’ collected in 2005, 2006 and 2007. The lines and arrows are put in place only as estimates of averages and to help illustrate the assumed trend in fractionation.

Nitrogen Cycling In Bison Pool: 2006 2006 Biofilm Bison N Story (J. Havig, in prep.)

Nitrogen Cycling In Bison Pool: 2006-2007 2006 Biofilm 2007 Biofilm Year-to-year variability? Bison N w/ 2007 Raleigh Distillation and N-fixation (J. Havig, in prep.)

Similar chemistry, similar isotopes Bison vs. Octopus Spring Bison vs. Spent Kleenex Similar chemistry, similar isotopes Bison vs. Octopus Spring Similar chemistry, different isotopes (J. Havig, in prep.)

Thank You Jeff Havig Dr. Ariel Anbar Stephen Romaniello Dr. Achim Herrmann Natasha Zolotova Dr. Everett Shock End: With thanks