JAZF1/JJAZ1 Gene Fusion in Endometrial Stromal Sarcomas

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JAZF1/JJAZ1 Gene Fusion in Endometrial Stromal Sarcomas Andelko Hrzenjak, Farid Moinfar, Fattaneh A. Tavassoli, Bettina- Strohmeier, Marie-Luise Kremser, Kurt Zatloukal, Helmut Denk The Journal of Molecular Diagnostics Volume 7, Issue 3, Pages 388-395 (August 2005) DOI: 10.1016/S1525-1578(10)60568-5 Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Amplification of JAZF1/JJAZ1 fusion transcript in 22 different ESSs. Nested PCR and RT-PCR were performed as described in Materials and Methods. A: Amplifications of large JAZF1/JJAZ1 transcripts (457 bases) were successful on RNA isolated from frozen tissue but not on RNA isolated from paraffin-embedded tissues. B: Small bands visible on the upper gel represent primer oligonucleotides. Amplification of the small transcript (93 bases) was successful on RNA isolations from frozen and formalin-fixed, paraffin-embedded tissues. Two high-grade UES cases are underlined. Frozen samples 23, 24, and 25 correspond to paraffin-embedded samples 10, 11, and 12, respectively. St., 100-bp DNA leader. The Journal of Molecular Diagnostics 2005 7, 388-395DOI: (10.1016/S1525-1578(10)60568-5) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 A–C: Examples of low-grade ESS with intense and diffuse immunoreaction for CD10. Note the infiltrative growth of the tumor into the surrounding myometrium (A) and a very strong (4+), membrane-associated positive staining (B). D–F: Examples of low-grade ESS with moderate (2+) and/or heterogeneous reactivity for CD10. Note heterogeneity of CD10 staining in different ESS samples. The Journal of Molecular Diagnostics 2005 7, 388-395DOI: (10.1016/S1525-1578(10)60568-5) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions