Differential stress sensitivity of the dividing versus non‐dividing cells. Differential stress sensitivity of the dividing versus non‐dividing cells. (A)

Slides:

Advertisements

Similar presentations

A stochastic feedback loop model predicts the kinetics of DDR and growth arrest at the single cell level. A stochastic feedback loop model predicts the.

Advertisements

Xiao-yu Zheng, Erin K. O’Shea Cell Reports

Cell death following DNA damage occurs from cells arrested in G1 or at the G1/S boundary. Cell death following DNA damage occurs from cells arrested in.

Bifurcation diagrams showing quasi‐steady‐state solutions and simulation of models. Bifurcation diagrams showing quasi‐steady‐state solutions and simulation.

Overview of the experimental setting.

Effects of ΔtolC on protein thermostability and abundance

Sensitivity of RNA‐seq.

Activity flow of the mTOR signaling network.

Predicted and measured double‐ring formation.

GFP Chlorophyll Merged A B C D

Notch levels in niche cells

Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.

TNF enhances dose‐dependent cell death following doxorubicin‐induced DNA damage with minimal affect on dose‐dependent cell‐cycle arrest. TNF enhances dose‐dependent.

Confirmation of pool data with isogenic culture

Dose response of pAGA1 and pFIG1 induction

Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent and cycling LGR5+ CRC cells Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent.

Projecting cellular immunophenotypes onto Drop‐seq data

Volume 5, Issue 6, Pages (December 2013)

Coupling immunophenotypes to Drop‐seq data

Characterization of promoters relative to pAGA1

Joseph M. Johnson, William J. Betz Biophysical Journal

Evaluating CRISPR negative selection screens.

Genomic profiling of fitness in periodic salt stress

Expression and occupancy of a set of transcription factors corresponding to the identified motifs at FAIRE peaks Expression and occupancy of a set of transcription.

Heat maps showing global relative growth phenotype and comparison between measured and predicted values. Heat maps showing global relative growth phenotype.

A Map for Horizontal Disparity in Monkey V2

Volume 22, Issue 12, Pages (March 2018)

DNA and membrane binding of MinD are interconnected.

RNAi causes widespread changes in cell population context leading to predictable changes in virus infection. RNAi causes widespread changes in cell population.

Mutants of the Group III have differentially impaired induction of pAGA1 and pFIG1 Mutants of the Group III have differentially impaired induction of pAGA1.

A Visual Framework for Classifying Determinants of Cell Size

A Super-Assembly of Whi3 Encodes Memory of Deceptive Encounters by Single Cells during Yeast Courtship Fabrice Caudron, Yves Barral Cell Volume 155,

Dynamic map of the Nap1p/Kcc4p interaction.

Volume 5, Issue 4, Pages e4 (October 2017)

Lag time to division depends on the frequency of pulsed glucose for a subpopulation Lag time to division depends on the frequency of pulsed glucose for.

Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.

Visualization of a global auxin‐response gradient in the root meristem

EB3 Regulates Microtubule Dynamics at the Cell Cortex and Is Required for Myoblast Elongation and Fusion Anne Straube, Andreas Merdes Current Biology

Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+

Bisection mapping of T7. RNAP

The cell cycle influences circadian phase progression Circadian intervals with divisions (p1,d1,p2) last 21.95 ± 3.8 h (n = 1,926) and are significantly.

Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6)‏ Computational wound healing gradients and in vivo.

Drug‐induced cell death and proliferation inhibition can be quantified from serial confocal images Drug‐induced cell death and proliferation inhibition.

Comparison of proteomics and RNA‐Seq data.

KIF18A sNL variants do not accumulate on K-fibers in the center of the spindle. KIF18A sNL variants do not accumulate on K-fibers in the center of the.

Direct role of HIS-24 and HPL in stress response.

Dynamics of induction of mating promoters after pheromone stimulation

Development of a 3D tissue model and in vivo live imaging during dermal maturation Development of a 3D tissue model and in vivo live imaging during dermal.

Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.

Subnetworks enriched for the hallmarks of cancer.

PKA mediates the primary transcriptional response of cells to glucose.

Stage‐dependent diurnal transcript changes.

Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.

Protein interactions at the nuclear pore.

Noise in hoxb1a/krox20 expression leads to boundary sharpening.

In vivo imaging of the wound bed at later time points (related to Fig 7)‏ In vivo imaging of the wound bed at later time points (related to Fig 7) A–CRepresentative.

Antisense expression associates with larger gene expression variability. Antisense expression associates with larger gene expression variability. (A–D)

Experimental validation of predicted endocytosis functions in human.

Time‐course analysis of NICD degradation.

Competence initiation during the progression to spore formation.

The transcriptional behavior of GREB1 changes with estrogen dose and exhibits considerable cell‐to‐cell variation (see also Fig EV2 and Movie EV2)‏ The.

Perturbation of ligand depletion affects the ultrasensitivity of long‐term P‐Smad2 responses to different doses of TGF‐β stimulation. Perturbation of ligand.

Microenvironment‐dependent differentiation and morphology.

(A) Observed significant protein fold‐changes during the fed–fasting transition in C57BL/6J (B6) and 129Sv (S9) mice fed with a normal diet (T0). (A) Observed.

Inhibitory effect of perhexiline on the proliferation of the HepG2 cell line A, BPerhexiline was used to mimic the effect of the l‐carnitine analog on.

Fraction of flux entering the PEP‐glyoxylate cycle as a function of hexose uptake rate in batch (A) and chemostat (B) cultures. Fraction of flux entering.

KIF18A sNL variants accumulate as well as WT on peripheral K-fibers.

Changes in fibroblast activation and proliferation outside the wound and CHP staining in the wound bed (related to Fig 5)‏ Changes in fibroblast activation.

Islet mass and β-cell proliferation is decreased in Hhip–/– embryos (Hip1 in figure). Islet mass and β-cell proliferation is decreased in Hhip–/– embryos.

Example recordings of an inward (A,C,E) and an outward left push (B,D,F) for subject 1. Example recordings of an inward (A,C,E) and an outward left push.

Presentation transcript:

Differential stress sensitivity of the dividing versus non‐dividing cells. Differential stress sensitivity of the dividing versus non‐dividing cells. (A) Mothers are more resistant to zinc shock. Following a period of 7 h in low zinc conditions, the cells were exposed to extremely high‐zinc conditions (SC medium supplemented with 9 mM Zn2+). The shift to high zinc concentration leads to a period of growth arrest followed by a recovery of the mothers. The daughters start recovering only 4 h later. Similar results were obtained when cells deleted of the vacuole membrane zinc storage transporter, Zrc1, were shifted to rich conditions. (B) Mothers are more resistant to osmotic stress. Following a period of 8.5 h in low zinc, the cells were shifted (t=0) to LZM with 150 mM NaCl and 1.2% Triton‐X100. Shown is the decline in the number of mothers and daughters. Note that 30% of the daughters compared with only 8% of the mothers imploded and died (see Supplementary Movies 3a and b for visualization of dying cells) during the time of the stress. (C, D) Daughters are more resistant to rapamycin. Following a period of 12 h in low zinc, the cells were exposed to rapamycin (16 μM) for 16 h, and then were resupplied with rich medium. The number of mothers and their progeny versus daughters and their progeny in one field of view, during the recovery stage in rich medium, is shown in (C). Note that while most mothers died (exploded), the daughters managed to recover (see Supplementary Movie 3c). The bar plots (bottom) show the percentage of dying and recovering cells in both populations averaged over four fields of view (∼100 cells). Error bars represent the s.d. of different fields of view from the mean value. Snapshots of a typical colony showing exploding mothers next to budding daughters, are shown in (D): the three mother cells (blue dot) marked by yellow arrow at t=5 h are shown to shrink/explode and to lose their nuclear marker (red) at t=8 h. The budding daughters (red dot) can be identified by the appearance of the bud neck (green). Nurit Avraham et al. Mol Syst Biol 2013;9:656 © as stated in the article, figure or figure legend