Volume 9, Issue 1, Pages (January 2002)

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Volume 9, Issue 1, Pages 25-33 (January 2002) Mutant Tyrosine Kinases with Unnatural Nucleotide Specificity Retain the Structure and Phospho-Acceptor Specificity of the Wild-Type Enzyme Laurie A. Witucki, Xin Huang, Kavita Shah, Yi Liu, Saw Kyin, Michael J. Eck, Kevan M. Shokat Chemistry & Biology Volume 9, Issue 1, Pages 25-33 (January 2002) DOI: 10.1016/S1074-5521(02)00091-1

Figure 1 Chemical Structures of A*TP Analogs Used in This Study 1: N6-(benzyl) ATP; 2: N6-(cyclopentyl) ATP. Definitions of analog-sensitive (as) kinase mutants used in this study. Chemistry & Biology 2002 9, 25-33DOI: (10.1016/S1074-5521(02)00091-1)

Figure 2 Comparison of Wild-Type and Analog-Specific c-Src Crystal Structures (A) The crystal structure of c-Src-as1 superimposed on wild-type c-Src. c-Src-as1 is shown in gray, and c-Src is in red. The rmsd for the overlay is 0.35 Å. (B) The binding of the A*TP analog, N6-(benzyl) ADP to the mutant c-Src (T338G) kinase. The surface corresponding to the glycine residue at the 338 position is colored red. The benzyl ring of the A*TP analog projects into a pocket in the nucleotide binding cleft. This pocket is made accessible by the c-Src (T338G) point mutation. For clarity, the 11 residues that bind over the nucleotide at the front of the nucleotide cleft are omitted from the figure in order to more clearly show the surface at the back of the nucleotide binding pocket where the 338 residue lies. The omitted residues are c-Src 272–282. (C) The steric clash of the wild-type c-Src threonine residue at the 338 position, shown in red, with the N6-(benzyl) ATP analog (blue). The gray surface was built over the crystal structure of the mutant kinase overlayed with the wild-type c-Src crystal structure, and the surface was rendered over threonine 338 (red). The N6-(benzyl) ADP (blue) is superimposed on the AMP-PNP ligand (yellow). Chemistry & Biology 2002 9, 25-33DOI: (10.1016/S1074-5521(02)00091-1)

Figure 3 Silver-Stain SDS-PAGE (12%) of the v-Src Kinases Used in This Study Lane 1, wild-type GST-v-Src (XD-4 construct: XD-4 [truncated v-Src kinase including the full catalytic domain, 77–225]); lane 2, GST-v-Src-as1; and lane 3, GST-v-Src-as2. Chemistry & Biology 2002 9, 25-33DOI: (10.1016/S1074-5521(02)00091-1)

Figure 4 Specificity Profile of Wild-Type and Analog-Specific v-Src with N6-(Benzyl) ATP The specificity profile of the v-Src-as2 mutant with N6-(benzyl) ATP is shown in gray. It is compared to the wild-type v-Src/ATP system, shown in black. Chemistry & Biology 2002 9, 25-33DOI: (10.1016/S1074-5521(02)00091-1)

Figure 5 Specificity Profile of Wild-Type and Analog-Specific v-Src with N6-(Cyclopentyl) ATP The specificity profile of the v-Src-as1 mutant with the A*TP analog N6-(cyclopentyl) ATP is shown in gray. It is compared to the wild-type v-Src/ATP system, shown in black. Chemistry & Biology 2002 9, 25-33DOI: (10.1016/S1074-5521(02)00091-1)

Figure 6 The Specificity Profile of the Src Family Kinase Fyn. The mutant Fyn-as1 tested with N6-(benzyl) ATP is shown in gray. It is compared to the wild-type Fyn/ATP system, shown in black. Chemistry & Biology 2002 9, 25-33DOI: (10.1016/S1074-5521(02)00091-1)