pTARGET1 pSWITCH1 pTARGET2 pSWITCH2 ampR PGAL1 yEGFP-CLN2(PEST) TRP1

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pTARGET1 pSWITCH1 pTARGET2 pSWITCH2 ampR PGAL1 yEGFP-CLN2(PEST) TRP1 CEN6/ARSH ADH1term pTARGET1 ampR PGAL1 URA3 f1 CEN6/ARSH ADH1term pSWITCH1 antiswitch ribozyme ampR PGAL1 TRP1 f1 CEN6/ARSH ADH1term pTARGET2 yEGFP-CLN2(PEST) Venus f1 ampR PGAL1 URA3 CEN6/ARSH ADH1term pSWITCH2 antiswitch 1 ribozyme antiswitch 2 Supplementary Figure 5 Set of plasmids used in the in vivo antiswitch characterization studies. pTARGET1 and pSWITCH1 were used in single antiswitch-single target studies and pTARGET2 and pSWITCH2 were used in multiple antiswitch-multiple target studies. All constructs are under the control of an inducible galactose promoter, contain a yeast nutrient-based selection marker, a yeast centromeric origin of replication, an E. coli f1 origin of replication, and an E. coli ampicillin resistance marker.