Stationary biofilm growth normalizes mutation frequencies and mutant prevention concentrations in Pseudomonas aeruginosa from cystic fibrosis patients

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Stationary biofilm growth normalizes mutation frequencies and mutant prevention concentrations in Pseudomonas aeruginosa from cystic fibrosis patients M. García‐Castillo, R. del Campo, F. Baquero, M.‐I. Morosini, M.‐C. Turrientes, J. Zamora, R. Cantón Clinical Microbiology and Infection Volume 17, Issue 5, Pages 704-711 (May 2011) DOI: 10.1111/j.1469-0691.2010.03317.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 UPGMA dendrogram with the generic relationship among the 42 Pseudomonas aeruginosa cystic fibrosis isolates and the control strains. NT, non‐typeable. Clinical Microbiology and Infection 2011 17, 704-711DOI: (10.1111/j.1469-0691.2010.03317.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Distribution of the mutation frequencies of all isolates in both planktonic and biofilm conditions. Arrows indicate major changes in mutation frequencies from the planktonic to the biofilm mode of growth. The convergence of hypermutable and hypomutable isolates towards normal mutation frequencies is presented. Bars indicate the number of hypomutators (grey), normomutators (white) and hypermutators (black) classified as having a planktonic mode of growth. Clinical Microbiology and Infection 2011 17, 704-711DOI: (10.1111/j.1469-0691.2010.03317.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Mutation frequencies for the 42 clinical isolates and the two control strains (PAO1, represented as a triangle, and PAO1ΔmutS, represented as a square) in planktonic conditions (black) (a), biofilm (grey) (b), and both modes of growth (c), and the difference between planktonic values and biofilm values (d). Clinical Microbiology and Infection 2011 17, 704-711DOI: (10.1111/j.1469-0691.2010.03317.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 4 Median MIC values in the planktonic (white) and biofilm (grey) conditions. Clinical Microbiology and Infection 2011 17, 704-711DOI: (10.1111/j.1469-0691.2010.03317.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions