Volume 6, Issue 10, Pages (October 1998)

Slides:



Advertisements
Similar presentations
Munirathinam Sundaramoorthy, James Terner, Thomas L Poulos  Structure 
Advertisements

Volume 95, Issue 7, Pages (December 1998)
The structure of OmpF porin in a tetragonal crystal form
Volume 21, Issue 1, Pages (January 2013)
Moses Prabu-Jeyabalan, Ellen Nalivaika, Celia A. Schiffer  Structure 
Volume 14, Issue 3, Pages (March 2001)
Volume 6, Issue 10, Pages (October 1998)
Volume 124, Issue 1, Pages (January 2006)
Volume 3, Issue 9, Pages (September 1995)
Volume 5, Issue 1, Pages (January 1997)
Volume 23, Issue 1, Pages (January 2015)
Volume 3, Issue 12, Pages (December 1995)
Crystal structure of human mitochondrial NAD(P)+-dependent malic enzyme: a new class of oxidative decarboxylases  Yingwu Xu, Girija Bhargava, Hao Wu,
Structural basis of inhibitor selectivity in MAP kinases
Mark Ultsch, Nathalie A Lokker, Paul J Godowski, Abraham M de Vos 
Volume 5, Issue 3, Pages (March 1997)
Hydration and DNA Recognition by Homeodomains
Volume 2, Issue 6, Pages (June 1994)
The three-dimensional structure of PNGase F, a glycosyl asparaginase from Flavobacterium meningosepticum  Gillian E Norris, Timothy J Stillman, Bryan.
Volume 8, Issue 3, Pages (March 2000)
Catalytic Center Assembly of HPPK as Revealed by the Crystal Structure of a Ternary Complex at 1.25 Å Resolution  Jaroslaw Blaszczyk, Genbin Shi, Honggao.
Volume 2, Issue 1, Pages (July 1998)
A biosynthetic thiolase in complex with a reaction intermediate: the crystal structure provides new insights into the catalytic mechanism  Yorgo Modis,
Volume 7, Issue 2, Pages (February 1999)
Influence of Protein Scaffold on Side-Chain Transfer Free Energies
Volume 6, Issue 12, Pages (December 1998)
Crystal Structure of PMM/PGM
The structural basis for pyrophosphatase catalysis
N Khazanovich, KS Bateman, M Chernaia, M Michalak, MNG James  Structure 
Volume 4, Issue 5, Pages (November 1999)
Volume 16, Issue 10, Pages (October 2008)
Munirathinam Sundaramoorthy, James Terner, Thomas L Poulos  Structure 
Volume 6, Issue 10, Pages (October 1998)
Volume 8, Issue 1, Pages (January 2001)
The 1.8 Å crystal structure of catechol 1,2-dioxygenase reveals a novel hydrophobic helical zipper as a subunit linker  Matthew W Vetting, Douglas H Ohlendorf 
Structure of the Catalytic Domain of Human DOT1L, a Non-SET Domain Nucleosomal Histone Methyltransferase  Jinrong Min, Qin Feng, Zhizhong Li, Yi Zhang,
Conformational changes on substrate binding to methylmalonyl CoA mutase and new insights into the free radical mechanism  Filippo Mancia, Philip R Evans 
The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica  Jonas Uppenberg, Mogens Trier Hansen,
Crystal Structure of Carnitine Acetyltransferase and Implications for the Catalytic Mechanism and Fatty Acid Transport  Gerwald Jogl, Liang Tong  Cell 
Volume 3, Issue 5, Pages (May 1999)
Antonina Roll-Mecak, Chune Cao, Thomas E. Dever, Stephen K. Burley 
Volume 10, Issue 6, Pages (June 2002)
The basis for K-Ras4B binding specificity to protein farnesyl-transferase revealed by 2 Å resolution ternary complex structures  Stephen B Long, Patrick.
The structure of an RNA dodecamer shows how tandem U–U base pairs increase the range of stable RNA structures and the diversity of recognition sites 
Masaru Goto, Rie Omi, Noriko Nakagawa, Ikuko Miyahara, Ken Hirotsu 
Volume 17, Issue 10, Pages (October 2009)
Volume 94, Issue 8, Pages (April 2008)
Volume 8, Issue 4, Pages (April 2000)
Volume 4, Issue 5, Pages (May 1996)
Crystal Structure of Imidazole Glycerol Phosphate Synthase
How glutaminyl-tRNA synthetase selects glutamine
Volume 7, Issue 8, Pages (August 1999)
Rebekka M. Wachter, S. James Remington  Current Biology 
Amedeo Caflisch, Martin Karplus  Structure 
Volume 5, Issue 10, Pages (October 1997)
Carl C. Correll, Betty Freeborn, Peter B. Moore, Thomas A. Steitz  Cell 
Structure of a water soluble fragment of the ‘Rieske’ iron–sulfur protein of the bovine heart mitochondrial cytochrome bc1 complex determined by MAD phasing.
T Barrett, CG Suresh, SP Tolley, EJ Dodson, MA Hughes  Structure 
Volume 5, Issue 10, Pages (October 1997)
Human glucose-6-phosphate dehydrogenase: the crystal structure reveals a structural NADP+ molecule and provides insights into enzyme deficiency  Shannon.
Volume 3, Issue 12, Pages (December 1995)
Structure of a HoxB1–Pbx1 Heterodimer Bound to DNA
Peter König, Rafael Giraldo, Lynda Chapman, Daniela Rhodes  Cell 
Structure of the Oxygen Sensor in Bacillus subtilis
Volume 6, Issue 8, Pages (August 1998)
Volume 126, Issue 4, Pages (August 2006)
Volume 5, Issue 6, Pages (June 1997)
Structural Basis for Activation of ARF GTPase
Volume 7, Issue 2, Pages (February 1999)
Stanley J Watowich, John J Skehel, Don C Wiley  Structure 
Presentation transcript:

Volume 6, Issue 10, Pages 1267-1277 (October 1998) Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein  Rebekka M Wachter, Marc-André Elsliger, Karen Kallio, George T Hanson, S James Remington  Structure  Volume 6, Issue 10, Pages 1267-1277 (October 1998) DOI: 10.1016/S0969-2126(98)00127-0

Figure 1 Normalized absorbance and fluorescence intensities of the YFP variant in 75 mM phosphate or acetate, and in 140 mM NaCl. Solid lines, absorbance scans at pH 6.0 (top scan at 400 nm), pH 7.0 (middle scan at 400 nm), and pH 8.0 (bottom scan at 400 nm); dashed line, the YFP fluorescence-emission scan at pH 7.0 (excitation wavelength 514 nm). Structure 1998 6, 1267-1277DOI: (10.1016/S0969-2126(98)00127-0)

Figure 2 Stereoview of the 2Fo–Fc electron-density map of the YFP chromophore and the stacked Tyr203 after refinement. The 2.5 å resolution map was contoured at +1 standard deviation. Structure 1998 6, 1267-1277DOI: (10.1016/S0969-2126(98)00127-0)

Figure 3 Stereo drawing of the chromophore showing residues 65, 68, 203, and backbone atoms of residues 61–64, 69 and 70. Green, S65T; yellow, YFP; orange, YFP H148G. The α carbons of the entire structures were overlayed (see text). Structure 1998 6, 1267-1277DOI: (10.1016/S0969-2126(98)00127-0)

Figure 4 Schematic diagram showing the immediate chromophore environment of (a) YFP and (b) YFP H148G. The proposed positions of hydrogen bonds are shown as dashed lines. Structure 1998 6, 1267-1277DOI: (10.1016/S0969-2126(98)00127-0)

Figure 5 Stereoview of the YFP chromophore and its environment. Oxygen atoms are shown in red, nitrogen atoms in blue, and carbon atoms in gray. Structure 1998 6, 1267-1277DOI: (10.1016/S0969-2126(98)00127-0)

Figure 6 Overlay of the backbone-atom trace of YFP (yellow) and YFP H148G (green). Part of the β barrel and the β-strand bulge around His148 are shown. The dashed lines represent possible hydrogen bonds. Structure 1998 6, 1267-1277DOI: (10.1016/S0969-2126(98)00127-0)

Figure 7 Stereoview of the solvent-accessible surface of YFP H148G, calculated using a 1.4 å probe radius (see text). The surface (cyan) was calculated after deleting the chromophore (magenta) and all water molecules (red). The outer surface of the protein is along the left edge of the figure. Internal cavities appear as appendages to the chromophore cavity within the right half of the figure (see text). Structure 1998 6, 1267-1277DOI: (10.1016/S0969-2126(98)00127-0)