Jieqiang Lv, M. D. , Xueqiong Zhu, M. D. , Ph. D

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Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics Jieqiang Lv, M.D., Xueqiong Zhu, M.D., Ph.D., Ke Dong, MS, Yi Lin, M.S., Yue Hu, M.D., Chundan Zhu, M.D. Fertility and Sterility Volume 90, Issue 5, Pages 1892-1898 (November 2008) DOI: 10.1016/j.fertnstert.2007.08.039 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Two-dimensional electrophoresis map of normal myometrium (N) and uterine leiomyoma (T), using a pH 3–8 immobilized pH gradient linear strip in the first dimension and a 200 mm × 185 mm × 1.0 mm 12% sodium dodecyl sulfate–polyacrylamide gel in the second dimension. The protein loading was 150 μg, and the gel was stained with the Silver Stain Plus kit from Bio-Rad. Fertility and Sterility 2008 90, 1892-1898DOI: (10.1016/j.fertnstert.2007.08.039) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Identification of 14-3-3 gamma down-regulation in leiomyoma tissue. (A) One of the protein spots with a consistent more than fivefold down-regulation in the leiomyoma specimens (arrow). N = normal myometrium; T = uterine leiomyoma. (B) Mass spectrometry of in-gel trypsin digests of this protein and analysis of the depicted peptide spectrum resulted in the identification of 14-3-3 gamma. Fertility and Sterility 2008 90, 1892-1898DOI: (10.1016/j.fertnstert.2007.08.039) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Western blot analysis revealed markedly down-regulated 14-3-3 gamma expression in leiomyoma samples (T) compared with that in normal (N) myometrium tissues. Depicted are 6 individual-matched pairs of normal myometrium and leiomyoma tissue samples. Fertility and Sterility 2008 90, 1892-1898DOI: (10.1016/j.fertnstert.2007.08.039) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Comparative 14-3-3 gamma reverse transcriptase polymerase chain reaction (RT-PCR) of individual-matched normal myometrium (N) and leiomyoma (T) tissues. In line with the results at the protein level, the RT-PCR results revealed marked down-regulation of 14-3-3 gamma in leiomyoma tissues compared with individual-matched normal myometrium tissue. Depicted are four individual- matched pairs of normal myometrium and leiomyoma tissue samples. Fertility and Sterility 2008 90, 1892-1898DOI: (10.1016/j.fertnstert.2007.08.039) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions