Analysis of complex DNA mixtures using the Forensim package

Slides:



Advertisements
Similar presentations
The most efficient STR loci in forensic genetics in population of central Poland R. Jacewicz, M. Jedrzejczyk, J. Berent Forensic Science International:
Advertisements

Exploring the relative DNA contribution of first and second object’s users on mock touch DNA mixtures  F. Oldoni, V. Castella, D. Hall  Forensic Science.
A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F
Activity level in the hierarchy of propositions in case of the People of the State of California v. Orenthal James Simpson analyzed using Bayesian network 
From sub-source to source: Interpreting results of biological trace investigations using probabilistic models  Wessel T. Oosterman, Bas Kokshoorn, Petra.
Use of matrix standards for new fluorophores in capillary sequencers
R. Alghafri, W. Goodwin, S. Hadi 
Statistical model for degraded DNA samples and adjusted probabilities for allelic drop- out  Torben Tvedebrink, Poul Svante Eriksen, Helle Smidt Mogensen,
A priori probabilities in Y23 mixture analysis: Non-contributor experiments using simulated powerplex® Y23 Y-STR mixtures  D. Moore, T. Clayton, J. Thomson 
Performance of the RapidHIT™200
A. Barbaro, P. Cormaci, G. Falcone 
Forensic identification of 12 mammals species based on size variation of mitochondrial cytochrome b gene using multiplex PCR assay  M. Črček, K. Drobnič 
Probabilistic modelling for DNA mixture analysis
Efficiency of DNA IQ System® in recovering semen DNA from cotton swabs
C. Davies, J. Thomson, F. Kennedy 
E.M. Dauber, B. Glock, W.R. Mayr 
The effect of increased cycle numbers using the Quantifiler® Duo DNA Quantification Kit (AB) on the detection of minute amounts of male DNA in mixtures.
Two mothers and two fathers for two half-brothers’ pairs
DNA typing of trace DNA recovered from different areas of sandals found at a homicide crime scene investigation: A comparative study  Samuel T.G. Ferreira,
Juliana G. Aquino, Juliana Jannuzzi, Elizeu F. Carvalho, Leonor Gusmão 
Establishment of Italian national DNA database and the central laboratory: Some aspects  R. Biondo, F. De Stefano  Forensic Science International: Genetics.
A new approach for the separation of spermatozoa from other cell types in forensically relevant samples  F. Grosjean, V. Castella  Forensic Science International:
Yan Liu, Li Li, Chengtao Li, Zhenmin Zhao 
A custom software solution for forensic mtDNA analysis of MiSeq data
R. Alghafri, S. Alhammadi, K. Amiri 
Population data for SE33 locus in United Arab Emirates Arab population
Automated extraction of DNA from clothing
Combining DNA evidence for greater match information
Oskar Hansson, Peter Gill 
J. Hedman, L. Albinsson, L. Norén, R. Ansell 
False homozygosity at D12S391 locus: A case report
A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F
On the meaning of the likelihood ratio: Is a large number always an indication of strength of evidence?  Hinda Haned, Guro Dørum, Thore Egeland, Peter.
Efficient DNA extraction from hair shafts
Evaluating DNA profiles using peak heights, allowing for dropin, dropout and stutters  Roberto Puch-Solis  Forensic Science International: Genetics Supplement.
Joanne L. Simons, Amelia Gamblin, Laura Arnold, Sue C. Vintiner 
DIP–STR: A new marker for resolving unbalanced DNA mixtures
A review of low template STR analysis in casework using the DNA SenCE post-PCR purification technique  T. Gross, J. Thomson, S. Kutranov  Forensic Science.
Split DNA over replicates or perform one amplification?
Introducing a latent variable approach for finding populations in a forensic DNA database  Maarten Kruijver  Forensic Science International: Genetics.
Determination of siblings: A special case report from Halle
C.J. Bruijning-van Dongen, K. Slooten, W. Burgers, W. Wiegerinck 
On the exact distribution of the numbers of alleles in DNA mixtures
Direct STR typing from bullet casings
Testing the behavior of GlobalFiler® PCR amplification kit with degraded and/or inhibited biological samples  V. Bogas, M. Carvalho, F. Corte-Real, M.J.
M. Hara, T. Masuda, A. Takada, T. Miyazaki, H. Suzuki, A. Kido, K
Is an increased drop-in rate appropriate with enhanced DNA profiling?
Semen detection: A retrospective overview from 2010
Linda Albinsson, Johannes Hedman, Ricky Ansell 
Kpop: A Python package for population genetics analysis
Simulated approach to estimate the number and combination of known/unknown contributors in mixed DNA samples using 15 short tandem repeat loci  Sho Manabe,
Sho Manabe, Yuya Hamano, Chihiro Kawai, Chie Morimoto, Keiji Tamaki 
When the alleged father is a close relative of the real father: The utility of insertion/deletion polymorphisms  M. Magalhaes, N. Pinto, C. Gomes, R.
mixsep: An R-package for DNA mixture separation
Interpretation of complex DNA profiles using Tippett plots
Georgina E. Meakin, Emma V. Butcher, Roland A. H. van Oorschot, Ruth M
Monique Wesselink, Irene Kuiper 
Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples  S. Gino, M. Omedei  Forensic Science.
Dmitriy A. Khodakov, Anastasia S. Khodakova, Adrian Linacre, Amanda V
Improved performance for forensic casework: Extraction and isolation updates for the Maxwell® 16 instrument  M. Lindner, P.V. Mandrekar, J. Bessetti,
Genetic data for the locus SE33 in a Southern Italy population with AmpFlSTR NGM SElect™ PCR amplification kit  A. Barbaro, P. Cormaci, A. La Marca  Forensic.
Development of a 24-plex Y chromosomal STR loci typing system
Statistical aspects of familial searching
L. Zha, L.B. Yun, H.B. Luo, J. Yan, Y.P. Hou 
S. Köhnemann, J. Kretzschmann, K. Mittmann, H. Pfeiffer 
Forensic nomenclature for short tandem repeats updated for sequencing
Low copy number typing has yet to achieve “general acceptance”
Discrepancies between forensic DNA databases
A.M. Ölçen, G. Filoğlu, H. Altunçul, S. Erdem, Ö. Bülbül 
A simple unconstrained semi-continuous model for calculating likelihood ratios for complex DNA mixtures  Daniel Russell, William Christensen, Todd Lindsey 
Presentation transcript:

Analysis of complex DNA mixtures using the Forensim package Hinda Haned, Peter Gill  Forensic Science International: Genetics Supplement Series  Volume 3, Issue 1, Pages e79-e80 (December 2011) DOI: 10.1016/j.fsigss.2011.08.039 Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Profiles of suspect, victim and a crime stain is provided. The dropped out alleles are highlighted in bold in the suspect's profile. Hatches represent victim's alleles and grey shading in the crime stain are shared alleles. Forensic Science International: Genetics Supplement Series 2011 3, e79-e80DOI: (10.1016/j.fsigss.2011.08.039) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Data input. The contributors under prosecution and defence propositions are highlighted by ‘tick boxes’. In this analysis the victim is conditional under both Hp and Hd propositions. The numbers of unknown contributors under each hypothesis, along with the model parameters are input. Forensic Science International: Genetics Supplement Series 2011 3, e79-e80DOI: (10.1016/j.fsigss.2011.08.039) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 (A) breakdown of conditional LR's per locus and combined LR for the profile. (B) Sensitivity analysis showing LR vs. PrD across the range 0–1. Forensic Science International: Genetics Supplement Series 2011 3, e79-e80DOI: (10.1016/j.fsigss.2011.08.039) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions