Students Get handout - FRQs LL mid-point Monday Food & drinks off of tables Phones in bin…muted or off…please & thank you

Electron transport chain Figure 10.13 How noncyclic electron flow during the light reactions generates ATP and NADPH P700 + CO2 Photosystem II (PS II) H2O Light LIGHT REACTIONS CALVIN CYCLE O2 NADPH [CH2O] (sugar) e Primary acceptor 2 H+ 1⁄2 1 Energy of electrons Pq Cytochrome complex Pc ATP Electron transport chain NADP+ Photosystem I (PS I) 6 2 ADP 5 Fd Electron Transport chain 7 reductase + 2 H+ 8 + H+ 4 e e O2 3 Light P680 1

Lab 4: Photosynthesis Dye-reduction technique….. -Tests that light & chloroplasts are needed for light reactions to occur DPIP replaces NADP+ as the final electron acceptor DPIP becomes when reduced (gaining electrons) Color change can be measured with spectrophotometer Experiment – write this summary down!! Set wavelength to 605 – check spec filter Prepare cuvettes from Table 4.3 (Don’t add chloroplasts….yet) Blank spec – read instructions next to wavelength knob Add chloroplasts & invert 2X to mix Place in spec & record for time 0 minutes Place in front of light Record at 5 minute intervals (get chloroplasts on ice please) Lab write-up - due WEDNESDAY just what is on the sheet….no pre-lab, purpose, procedure

Unboiled Chloroplasts, Dark 3 Unboiled, Chloroplasts, Light 4 Cuvettes 1 Blank 2 Unboiled Chloroplasts, Dark 3 Unboiled, Chloroplasts, Light 4 Boiled, Chloroplasts, 5 No Chloroplasts Phosphate Buffer 1 mL dH2O 4 mL 3 mL 3 mL + 3 drops DPIP Unboiled Chloroplasts _______ Boiled Chloroplasts 20 drops = 1 mL Add chloroplasts LAST