Efficacy of Therapeutic Angiogenesis by Intramyocardial Injection of pCK-VEGF165 in Pigs Jae-Sung Choi, MD, PhD, Ki-Bong Kim, MD, PhD, Woong Han, DVM,

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Efficacy of Therapeutic Angiogenesis by Intramyocardial Injection of pCK-VEGF165 in Pigs Jae-Sung Choi, MD, PhD, Ki-Bong Kim, MD, PhD, Woong Han, DVM, Dong Sik Kim, DVM, Jin Sik Park, MD, Jong Jin Lee, MD, Dong Soo Lee, MD, PhD The Annals of Thoracic Surgery Volume 82, Issue 2, Pages 679-686 (August 2006) DOI: 10.1016/j.athoracsur.2006.03.028 Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Selection of 10 subject segments for segmental analysis in 20-segment model. (LAD = left anterior descending coronary artery; LCX = left circumflex coronary artery; RCA = right coronary artery.) The Annals of Thoracic Surgery 2006 82, 679-686DOI: (10.1016/j.athoracsur.2006.03.028) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Serial single-photon emission computed tomographic myocardial perfusion imaging showed that perfusion defects in the apical and anteroseptal walls and a perfusion decrease in the lateral wall on day 30 (A) improved substantially on day 60 (B). The Annals of Thoracic Surgery 2006 82, 679-686DOI: (10.1016/j.athoracsur.2006.03.028) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Comparison of segmental perfusion (A), wall thickening (B), and wall motion (C) measured by gated myocardial single-photon emission computed tomography on day 30 (white bars) and day 60 (hatched bars). (VEGF = vascular endothelial growth factor.) The Annals of Thoracic Surgery 2006 82, 679-686DOI: (10.1016/j.athoracsur.2006.03.028) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 (A) More microvessels stained by alkaline phosphatase were observed in the vascular endothelial growth factor (VEGF) group than in the control group (original magnification, ×200). (B) Comparison of the numbers of microvessels between the two groups. The Annals of Thoracic Surgery 2006 82, 679-686DOI: (10.1016/j.athoracsur.2006.03.028) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions