In Vitro Expansion and Transplantation of Intestinal Crypt Stem Cells Vivian S.W. Li, Hans Clevers  Gastroenterology  Volume 143, Issue 1, Pages 30-34 (July 2012) DOI: 10.1053/j.gastro.2012.05.017 Copyright © 2012 AGA Institute Terms and Conditions

Figure 1 In vitro expansion of human colonic organoids from a single stem cell. Representative colon spheroid derived from a single EPHB2high human colonic crypt cell from day 0 to day 13. Scale bar, 50 μm. (Reprinted with permission from Jung et al.23) Gastroenterology 2012 143, 30-34DOI: (10.1053/j.gastro.2012.05.017) Copyright © 2012 AGA Institute Terms and Conditions

Figure 2 Colon organoid transplantation. (A) Colon organoid cartoon with crypt and differentiated-domain. (B, C) Expanded organoids are introduced into the colon damaged by dextran sulphate sodium. (D) Transplanted organoids eventually fuse to cover the lesion. (E, F) Confocal image of long-term engrafted (6 months) clonal organoids derived from an RFP+ donor mouse into RFP− recipient mouse. Of note, transplanted organoids form complete colonic crypts with all cell lineages.24 Images are shown with (F) or without (E) 4′,6-diamidino-2-phenylindole staining. (Courtesy of Mamoru Watanabe and Tetsuya Nakamura). Gastroenterology 2012 143, 30-34DOI: (10.1053/j.gastro.2012.05.017) Copyright © 2012 AGA Institute Terms and Conditions