Effects of Lp(a) Treatment on HAVICs HAVICs were incubated in OSM in the presence or absence of 50 μg/ml purified Lp(a) (Athens Research & Technology,

Slides:

Advertisements

Similar presentations

Lab 2: Organelles, Proteins, and SDS-PAGE

Advertisements

Results 1.Miller JR, Siripurkpong P, Hawes J, Majdalawieh A, Ro HS, McLeod RS. The trans-10, cis-12 isomer of conjugated linoleic acid decreases adiponectin.

PreimmuneMonoclonalanti-Aur-AAffinity Purified anti-Aur-A Blot: retic lysate translation product oocyte extract retic lysate translation.

Volume 68, Issue 4, Pages (October 2005)

Increase in Elastin Gene Expression and Protein Synthesis in Arterial Smooth Muscle Cells Derived From Patients with Moyamoya Disease by Mari Yamamoto,

Induction of Apoptosis by Ethanol Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells Kyung-Jun Jang, Min-Ho Han, Byung-Hoon Lee, Byung-Woo.

Suppressive Effects of Cyclosporin A and FK-506 on Superoxide Generation in Human Polymorphonuclear Leukocytes Primed by Tumor Necrosis Factor α Yasushi.

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

STAT3-mediated constitutive expression of SOCS-3 in cutaneous T-cell lymphoma by Christine Brender, Mette Nielsen, Keld Kaltoft, Gitte Mikkelsen, Qian.

Volume 140, Issue 3, Pages (March 2011)

by Christina K. Ullrich, Jerome E. Groopman, and Ramesh K. Ganju

von Willebrand factor is a cofactor in complement regulation

SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI cross. SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI

S100A7 (Psoriasin) – Mechanism of Antibacterial Action in Wounds

Chronic neutropenia mediated by Fas ligand

Volume 60, Issue 1, Pages (July 2001)

Characterization of proteinuria and tubular protein uptake in a new model of oral L-lysine administration in rats K. Thelle, E.I. Christensen, H. Vorum,

Bone morphogenetic protein-2 induces apoptosis in human myeloma cells with modulation of STAT3 by Chiharu Kawamura, Masahiro Kizaki, Kenji Yamato, Hideo.

Pnut is phosphorylated during early Drosophila embryogenesis.

LPA mRNA and Protein Expression Were Detected in Primarily Cultured HAVICs and Aortic Valve Tissues (A) RT-PCR detection of LPA mRNA expression in primarily.

Volume 64, Issue 2, Pages (August 2003)

Effects of Lp(a) on Inorganic Mineralization in HAVICs (A) Representative Raman spectra. Noncalcified HAVICs were incubated in OSM in the absence (a) or.

An Autocrine Loop Mediates Expression of Vascular Endothelial Growth Factor in Human Dermal Microvascular Endothelial Cells Barbara Vega-Diaz, Serge.

Effects of Lp(a) on Cytokines and Phosphorylated MAPKs in HAVICs (A) Effects of Lp(a) on release of cytokines and chemokines by HAVICs. Rectangular outlines.

Evidence for low-density lipoprotein–induced expression of connective tissue growth factor in mesangial cells Mimi Sohn, Yan Tan, Richard L. Klein, Ayad.

Effects of Lp(a) on HAVIC Gel Contraction, β-Catenin Nuclear Translocation, and Lp(a) Uptake (A) Effect of Lp(a) treatment on gel contraction. Lp(a)-treated.

The absence of ADCC by nivolumab in vitro.

Immunostaining of LPA and OxPLs in Human Aortic Valve Tissues (A) Normal valve shows no obvious LPA immunoreactivity; aorta side on top (dashed arrow).

SDS-PAGE analysis of mature seed from lines Patriot and PI

Volume 68, Issue 4, Pages (October 2005)

Protein Phosphatase Type 1-Dependent Dephosphorylation of the Retinoblastoma Tumor Suppressor Protein in Ultraviolet-Irradiated Human Skin and Keratinocytes

Volume 29, Issue 2, Pages (February 2008)

Induction of Adipose Differentiation Related Protein and Neutral Lipid Droplet Accumulation in Keratinocytes by Skin Irritants Emmanuela Corsini, PhD,

1,25-dihydroxyvitamin D3 inhibits renal interstitial myofibroblast activation by inducing hepatocyte growth factor expression Yingjian Li, Bradley C.

Volume 64, Issue 1, Pages (July 2003)

Multiple distinct molecular mechanisms influence sensitivity and resistance to MDM2 inhibitors in adult acute myelogenous leukemia by Jianting Long, Brian.

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

Gangliosides Inhibit Urokinase-Type Plasminogen Activator (uPA)-Dependent Squamous Carcinoma Cell Migration by Preventing uPA Receptor/α5β1 Integrin/Epidermal.

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

Bcl-2 Reduced and Fas Activated by the Inhibition of Stem Cell Factor/KIT Signaling in Murine Melanocyte Precursors Satoko Kimura, Tamihiro Kawakami,

Md Nasimuzzaman, Danielle Lynn, Johannes CM van der Loo, Punam Malik

Eva Balint, Cory F. Marshall, Dr Stuart M. Sprague

Malondialdehyde oxidation of cartilage collagen by chondrocytes

Peroxisome proliferator-activated receptor-γ ligand reduced tumor necrosis factor-α- induced interleukin-8 production and growth in endometriotic stromal.

Volume 60, Issue 3, Pages (September 2001)

Effect of asymmetric dimethylarginine on osteoblastic differentiation

Titration of antisera against the amount of extract loaded on Western blots. Titration of antisera against the amount of extract loaded on Western blots.

Figure 2 Glatiramer acetate treatment induced M2 differentiation through a MyD88-independent pathway (A) As described previously,3 M2 monocytes were treated.

IRS-1 and -2 expression in four mesangial cell lines from different D-NOD (A and B) and ND-NOD (C and D) mice. IRS-1 and -2 expression in four mesangial.

Volume 77, Issue 5, Pages (March 2010)

The effect of purified IgG from healthy controls or patients with lupus nephritis on tyrosine phosphorylation in podocytes at two different IgG concentrations.

Arsenic Induces Human Keratinocyte Apoptosis by the FAS/FAS Ligand Pathway, Which Correlates with Alterations in Nuclear Factor-κB and Activator Protein-1.

Lipid Rafts and the Oxidative Stress Hypothesis

Active MT1-MMP is recycled to the cell surface in HT1080 cells.

MEL23 and MEL24 inhibit Mdm2 and p53 ubiquitination through the Mdm2-MdmX hetero-complex. MEL23 and MEL24 inhibit Mdm2 and p53 ubiquitination through the.

Fertility and Sterility

In vivo localization of Iff2, Iff3 (Iff9), Iff5, Iff6, Iff8, and Dcw1 proteins. In vivo localization of Iff2, Iff3 (Iff9), Iff5, Iff6, Iff8, and Dcw1 proteins.

Suppressive Effects of Cyclosporin A and FK-506 on Superoxide Generation in Human Polymorphonuclear Leukocytes Primed by Tumor Necrosis Factor α Yasushi.

The role of p53 in H2O2-mediated cell death of hOGG1-deficient H1299 lung carcinoma p53 null cells. The role of p53 in H2O2-mediated cell death of hOGG1-deficient.

AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2. AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2.

Western immunoblot of Acanthamoeba whole-cell lysates reacted with normal human serum, demonstrating immunoreactivity against Acanthamoeba antigens. Western.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

IL-13Rα2 promotes cell survival and proliferation.

Characterization of the complex formed at the Fra-1 RCE1.

Biological activity of IL-2 and GM-CSF in DCH and variants.

Ligation efficiency is abrogated with increasing concentration of antibodies against NHEJ proteins. Ligation efficiency is abrogated with increasing concentration.

Doxycycline Indirectly Inhibits Proteolytic Activation of Tryptic Kallikrein-Related Peptidases and Activation of Cathelicidin Kimberly N. Kanada, Teruaki.

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

Effect of MIF siRNA on the production of MMP-13 induced by LPA

Presentation transcript:

Effects of Lp(a) Treatment on HAVICs HAVICs were incubated in OSM in the presence or absence of 50 μg/ml purified Lp(a) (Athens Research & Technology, Inc., Athens, Georgia) for 48 h. (A) Cell proliferation measured using an MTT protocol and presented as relative cell proliferation rate. *p = 0.013 vs. OSM control. (B) Medium and cellular ALP activity measured with an ALP activity colorimetric assay kit and normalized to total protein concentration. Lp(a)-treated cells showed higher ALP activity in medium (**p = 0.0001) and cell lysate (*p = 0.003) than OSM control. (C) Medium phosphate release measured using a phosphate colorimetric assay kit (BioVision, Inc., Milpitas, California). *p = 0.0001 vs. OSM (control). (D) No detectable OxPLs were seen on purified Lp(a) using anti-OxPL E06 antibody. Purified human Lp(a) (1 μg) was loaded on 6% sodium dodecyl sulfate polyacrylamide gel electrophoresis. Lane 1: Lp(a) was detected using anti-Lp(a) antibody (diluted at 1:10,000; Novus Biologicals, LLC, Littleton, Colorado); lane 2: no OxPLs were detected in purified Lp(a) using E06 antibody (diluted at 1:1,000; Avanti Polar Lipids, Inc., Alabaster, Alabama). ALP = alkaline phosphatase; HAVICS = human aortic valve interstitial cells; Lp(a) = lipoprotein(a); OSM = osteogenic medium; OxPL = oxidized phospholipid. Bin Yu et al. BTS 2017;2:358-371 2017 The Authors