Agar media.

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Presentation transcript:

Agar media

Growth medium A growth medium or culture medium is a solid, liquid or semi-solid designed to support the growth of microorganisms The main types are - Cultural media - Minimal media - Selective media - Differential media

Unculturable bacteria Overall, it is estimated that only 0.1 to 1% of all soil bacteria can be cultured. In marine environments, the percentage of bacterial cells that can be recovered and cultured is even smaller, about 0.01 to 0.1 percent.

R2A agar (Reasoner's 2A agar) R2A agar (Reasoner's 2A agar) is a culture medium developed to study bacteria which normally inhabit potable water. These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium. Since its development in 1985,[3] it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media.

LB agar (Luria-Bertani medium) Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. The formula of the LB medium was published in 1951 in the first paper of Bertani on lysogeny. LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.[