Volume 7, Issue 6, Pages (June 2014)

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Volume 7, Issue 6, Pages 1053-1056 (June 2014) A Rapid Phytohormone and Phytoalexin Screening Method for Physiological Phenotyping Großkinsky Dominik K. , Albacete Alfonso , Jammer Alexandra , Krbez Peter , van der Graaff Eric , Pfeifhofer Hartwig , Roitsch Thomas Molecular Plant Volume 7, Issue 6, Pages 1053-1056 (June 2014) DOI: 10.1093/mp/ssu015 Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

Figure 1 Rapid Screening Method for Parallel Determination of Phytohormones and Phytoalexins. (A) Flow chart showing the individual steps of parallel phytohormone and phytoalexin extraction from the same source material in less than 5h. (B) Phytohormone and phytoalexin accumulation (ng g–1 FW) in A. thaliana and N. tabacum leaves 48h post infection with P. syringae. Mean values ± standard error of two independent biological experiments consisting of two technical replicates each. (C) Phytohormone accumulation (ng g–1 FW) in various plant material and cell suspension cultures. Mean values ± standard error of two independent biological experiments consisting of two technical replicates each. ABA, abscisic acid; CK, cytokinin; cZ, cis-zeatin; DHZ, dihydrozeatin; DHZR, dihydrozeatinriboside; IAA, indole-3-acetic acid; iP, isopentenyladenine; JA, jasmonic acid; ND, not detectable; SA, salicylic acid; tZ, trans-zeatin; tZOG, trans-zeatin-O-glucoside; tZR, trans-zeatinriboside; tZROG, trans-zeatinriboside-O-glucoside. Molecular Plant 2014 7, 1053-1056DOI: (10.1093/mp/ssu015) Copyright © 2014 The Authors. All rights reserved. Terms and Conditions