Lack of correlation between an assay used to determine early marrow allograft rejection and long-term chimerism after murine allogeneic bone marrow transplantation:

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Lack of correlation between an assay used to determine early marrow allograft rejection and long-term chimerism after murine allogeneic bone marrow transplantation: Effects of marrow dose Crystal Y. Koh, Lisbeth A. Welniak, William J. Murphy Biology of Blood and Marrow Transplantation Volume 11, Issue 4, Pages 252-259 (April 2005) DOI: 10.1016/j.bbmt.2005.01.006 Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 Overall survival and CFU-GM in spleens of recipients after allogeneic BMT. B6 recipients were lethally irradiated at 9.5 Gy and received various doses of BALB/c BMCs. A, At day 8 to 12 after BMT, splenocytes were harvested from 3 mice per group in each experiment and plated in semisolid media containing rmGM-CSF and rm interleukin 3 as described in Materials and Methods. The numbers represent mean ± SEM. Statistical differences between groups were determined by analysis of variance with the Tukey multiple comparison test. B, B6 mice (30 to 31 mice per group) that had undergone transplantation were monitored for survival. Pooled data from 3 independent experiments are shown, and statistical differences were determined by the log-rank test. Biology of Blood and Marrow Transplantation 2005 11, 252-259DOI: (10.1016/j.bbmt.2005.01.006) Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Delayed kinetics of hematopoietic cell recovery in peripheral blood in mice that received limited doses of BMC allografts. B6 mice were lethally irradiated at 9.5 Gy and injected with BALB/c BMCs (intravenously) at 1.5 × 107, 5 × 106, or 2.5 × 106 per mouse. Peripheral blood samples were collected weekly (3–5 mice per group per experiment) and analyzed for the levels of total WBCs, neutrophils, lymphocytes, red blood cells, and platelets recovered after BMT as described in Materials and Methods. Pooled data from 3 independent experiments are shown. Stars represent statistical differences compared with the group that received 1.5 × 107 BALB/c BMCs, as determined by analysis of variance with the Tukey multiple comparison test. Biology of Blood and Marrow Transplantation 2005 11, 252-259DOI: (10.1016/j.bbmt.2005.01.006) Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 The effect of dose of BMC allograft on long-term donor cell engraftment in the bone marrow and thymus. B6 mice were lethally irradiated at 9.5 Gy and injected with BALB/c BMCs (intravenously) at 1.5 × 107, 5 × 106, or 2.5 × 106 per mouse, and at day 90 after BMT, BMCs, splenocytes, and thymocytes were isolated from the recipients (2–7 mice per group per experiment). BMCs were stained with B-Gr-1 followed by Cy-SA and then with FITC-H2Dd and PE-H2Kb. Thymocytes were stained with B-H2Kb followed by Cy-SA and then with FITC-H2Dd and a mixture of PE-CD4 and PE-CD8. Splenocytes were stained with FITC-H2Dd and PE-H2Kb. Stained cells were analyzed with a FACScan cell analyzer using Cell Quest software. A pool of 2 independent experiments is shown, and the statistical differences between groups were determined by analysis of variance with the Tukey multiple comparison test. Biology of Blood and Marrow Transplantation 2005 11, 252-259DOI: (10.1016/j.bbmt.2005.01.006) Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 4 The effect of dose of BMC allograft on the percentage of donor cell chimerism. B6 mice were lethally irradiated at 9.5 Gy and injected with BALB/c BMCs (intravenously) at 1.5 × 107, 5 × 106, or 2.5 × 106 per mouse, and at day 90 after BMT, splenocytes were isolated from the recipients (2–7 mice per group per experiment). Donor- versus host-derived cells were determined by flow cytometric analysis as described in Materials and Methods. A pool of 2 independent experiments is shown, and the statistical differences between groups were determined by analysis of variance with the Tukey multiple comparison test. Biology of Blood and Marrow Transplantation 2005 11, 252-259DOI: (10.1016/j.bbmt.2005.01.006) Copyright © 2005 American Society for Blood and Marrow Transplantation Terms and Conditions